부산대학교 도서관

The most common nosocomial fungal infections are caused by several species of Candida, of which Candida glabratais the second most frequently isolated species from bloodstream infections. C. glabratadisplays relatively high minimal inhibitory concentration values (MIC) to the antifungal fluconazole and is associated with high mortality rates. To decrease mortality rates, the appropriate treatment must be administered promptly. C. glabratacontains in its genome several non-identical copies of species-specific sequences. We designed three pairs of C. glabrata-specific primers for endpoint PCR amplification that align to these species-specific sequences and amplify the different copies in the genome. Using these primers, we developed a fast, sensitive, inexpensive, and highly specific PCR-based method to positively detect C. glabrataDNA in a concentration-dependent manner from mixes of purified genomic DNA of several Candidaspecies, as well as from hemocultures and urine clinical samples. This tool can be used for positive identification of C. glabratain the clinic.