학술논문
Sequence of a Second Gene Encoding Bovine Submaxillary Mucin: Implication for Mucin Heterogeneity and Cloning
Document Type
Article
Source
Biochemical and Biophysical Research Communications; October 1998, Vol. 251 Issue: 2 p550-556, 7p
Subject
Language
ISSN
0006291X; 10902104
Abstract
Secreted epithelial mucins are extremely large and heterogeneous glycoproteins. We report the 5 kilobase DNA sequence of a second gene,BSM2,which encodes bovine submaxillary mucin. The determined nucleotide and deduced amino acid sequences ofBSM2are 95.2% and 92.2% identical, respectively, to those of the previously describedBSM1gene isolated from the same cow. Further, the five predicted protein domains of the two genes are 100%, 94%, 93%, 77%, and 88% identical. Based on the above results, we propose that expression of multiple homologous core proteins from a single animal is a factor in generating diversity of saccharides in mucins and in providing resistance of the molecules to proteolysis. In addition, this work raises several important issues in mucin cloning such as assembling sequences from seemingly overlapping clones and deducing consensus sequences for nearly identical tandem repeats.