학술논문
Plasmid instability in regenerating protoplasts of Staphylococcus aureus is caused by aberrant cell division
Document Type
Article
Source
Journal of Bacteriology; March 1986, Vol. 165 Issue: 3 p878-883, 6p
Subject
Language
ISSN
00219193; 10985530
Abstract
Elimination of plasmids from regenerating S. aureus protoplasts occurred when the regeneration medium contained sucrose but not when it contained sodium succinate. This difference was caused by the occurrence of cell division prior to regeneration of the cell wall on sucrose but not on succinate. Coexisting compatible plasmids were cured independently; coexisting incompatible plasmids were cured jointly. These results support the hypothesis that plasmid pools exist as physically sequestered units in protoplasts and that curing is a consequence of the segregation of such units during abnormal division of wall-less organisms.