학술논문
Expression and Self-Assembly of Norwalk Virus Capsid Protein from Venezuelan Equine Encephalitis Virus Replicons
Document Type
Article
Author
Source
The Journal of Virology; March 2002, Vol. 76 Issue: 6 p3023-3030, 8p
Subject
Language
ISSN
0022538X; 10985514
Abstract
ABSTRACTThe Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells.