학술논문
Metabolic determination of cell fate through selective inheritance of mitochondria
Document Type
Article
Author
Döhla, Julia; Kuuluvainen, Emilia; Gebert, Nadja; Amaral, Ana; Englund, Johanna I.; Gopalakrishnan, Swetha; Konovalova, Svetlana; Nieminen, Anni I.; Salminen, Ella S.; Torregrosa Muñumer, Rubén; Ahlqvist, Kati; Yang, Yang; Bui, Hien; Otonkoski, Timo; Käkelä, Reijo; Hietakangas, Ville; Tyynismaa, Henna; Ori, Alessandro; Katajisto, Pekka
Source
Nature Cell Biology; February 2022, Vol. 24 Issue: 2 p148-154, 7p
Subject
Language
ISSN
14657392; 14764679
Abstract
Metabolic characteristics of adult stem cells are distinct from their differentiated progeny, and cellular metabolism is emerging as a potential driver of cell fate conversions1–4. How these metabolic features are established remains unclear. Here we identified inherited metabolism imposed by functionally distinct mitochondrial age-classes as a fate determinant in asymmetric division of epithelial stem-like cells. While chronologically old mitochondria support oxidative respiration, the electron transport chain of new organelles is proteomically immature and they respire less. After cell division, selectively segregated mitochondrial age-classes elicit a metabolic bias in progeny cells, with oxidative energy metabolism promoting differentiation in cells that inherit old mitochondria. Cells that inherit newly synthesized mitochondria with low levels of Rieske iron–sulfur polypeptide 1 have a higher pentose phosphate pathway activity, which promotes de novo purine biosynthesis and redox balance, and is required to maintain stemness during early fate determination after division. Our results demonstrate that fate decisions are susceptible to intrinsic metabolic bias imposed by selectively inherited mitochondria.