부산대학교 도서관

ABSTRACTLactococcus garvieae(junior synonym Enterococcus seriolicida) is an emerging zoonotic agent isolated from economically important fish (rainbow trout and yellowtail), from cattle, and from humans. Clindamycin susceptibility is the only phenotypic test which can differentiate L. garvieaefromLactococcus lactis, another emerging agent in humans. A PCR assay for the identification of L. garvieaewas developed and resulted in an amplified fragment of 1,100 bp in size. The PCR assay was shown to be specific to L. garvieae. The PCR assay was positive for all the L. garvieaestrains tested, which originated from three different continents (Asia, Australia, and Europe). The PCR assay was negative for the phenotypically similarL. lactisand for all the other fish pathogens tested, including Streptococcus iniaeand Aeromonas salmonicida. The PCR assay was applied to plasma obtained from diseased animals and was found sensitive enough to detect bacteria from 1 µl of plasma. The PCR assay that was developed is the only practical test besides the clindamycin test which can specifically identify the zoonotic agent L. garvieaeand which can differentiate it from L. lactis.