부산대학교 도서관

Campylobacteris a major cause of human foodborne disease worldwide and has been associated with the consumption of contaminated poultry. The prevalence of Campylobacterspecies in broiler flocks from more than 200 producers widespread in mainland Portugal was assessed in 2008 in response to Commission Decision 2007/516/EC. Campylobacterisolates were obtained from 83.3% of 424 pooled cecal samples, with a higher prevalence of Campylobacter coli(61.2%) than Campylobacter jejuni(38.8%). Restriction fragment length polymorphism analysis of the flaAgene (flaA-RFLP)of 112 C. coliisolates and 67 C. jejuniisolates yielded 11 flaA-RFLP patterns with HinfI (Hunter Gaston diversity index [HGDI] = 0.62) and 48 flaA-RFLP patterns with DdeI (HGDI = 0.89), indicating a high level of genetic diversity. A wide geographic distribution of the most frequent restriction pattern was observed. MICs of five antimicrobials (ciprofloxacin, gentamicin, streptomycin, erythromycin, and tetracycline) were determined for 215 C. coliisolates and 136 C. jejuniisolates. The occurrence of non-wild-type isolates, exhibiting an acquired resistance phenotype, was higher for C. colithan C. jejunifor all antimicrobials tested. Sixty-three percent of C. jejuniand C. coliisolates were considered non-wild type based on their response to both ciprofloxacin and erythromycin, which are frequently used in the treatment of human infections. The high prevalence of antimicrobial-resistant Campylobacterstrains detected supports the need for increased epidemiological surveillance and prevention in a country where large amounts of poultry meat are consumed.