학술논문
Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids
Document Type
Article
Author
Source
Journal of Bacteriology; March 1990, Vol. 172 Issue: 3 p1436-1440, 5p
Subject
Language
ISSN
00219193; 10985530
Abstract
Conjugative temperature-sensitive plasmids were derived from pSC101. These plasmids are useful in genetic analysis for two reasons: (i) they render possible the construction of new Hfr lines by plasmid integration at predetermined chromosomal loci via Tn10 inverse transposition, and (ii) the Hfr characters are transducible via bacteriophage P1. We also showed that replication from pSC101 origin is deleterious for the plasmid-chromosome fusion.