학술논문
High-performance liquid chromatographic determination of piperacillin in plasma
Document Type
Article
Author
Source
Antimicrobial Agents and Chemotherapy; April 1982, Vol. 21 Issue: 4 p664-665, 2p
Subject
Language
ISSN
00664804; 10986596
Abstract
A high-performance liquid chromatographic method has been developed for the quantitative determination of piperacillin in plasma. The compound is first extracted from acid-treated plasma into chloroform: 1-pentanol (3:1) and reextracted into a small volume of an aqueous phase at pH 7, which is injected in the chromatographic column (microgram-Bondapack C18; 10 micrometers). The mobile phase is a mixture of 0.01 M acetate buffer (pH 4.8) and methanol. The method is accurate and reproducible with a sensitivity of about 50 ng of piperacillin per ml of serum.