부산대학교 도서관

In this study, we investigated the growth and extracellular matrix synthesis of human osteoblastlike cells on highly porous natural bone mineral. Human bone cells were isolated from trabecular bone during routine iliac crest biopsies. Under conventional culture conditions, trabecular bone cells were able to assume the organization of a threedimensional structure on a porous natural bone mineral BioOss® Block. Scanning electron microscopy examination after 6 weeks revealed multiple cell layers on the trabecular block. Transmission electron microscopy examination after 6 weeks revealed the accumulation of mature collagen fibrils in the intracellular and extracellular spaces, and showed multilayered, rough endoplasmic reticulum as well as mitochondriarich cells surrounded by dense extracellular matrix. These morphological observations suggest that the cell layer may resemble the natural threedimensional structure. Biochemical analysis revealed that the hydroxylysylpyridinoline, lysylpyridinoline, and hydroxyproline content of the cell layer increased in a timedependent manner, whereas in monolayer culture without natural bone mineral, no measurable amounts of hydroxylysylpyridinoline or lysylpyridinoline, and a barely measurable amount of hydroxyproline, were noted. Mature collagen extracted by ethylenediaminetetraacetic aciddemineralization from the cell layer on natural bone mineral showed an identical electrophoretic pattern to that observed in human bone, as evaluated by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. The present study demonstrated an excellent biocompatibility of the highly porous natural bone mineral in a threedimensional bone cell culture system, and thus its potential for tissueengineered growth of human bone. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 51, 703–710, 2000.