학술논문
A combinatorial genetic strategy for exploring complex genotype–phenotype associations in cancer
Document Type
Article
Author
Source
Nature Genetics; 20240101, Issue: Preprints p1-6, 6p
Subject
Language
ISSN
10614036; 15461718
Abstract
Available genetically defined cancer models are limited in genotypic and phenotypic complexity and underrepresent the heterogeneity of human cancer. Here, we describe a combinatorial genetic strategy applied to an organoid transformation assay to rapidly generate diverse, clinically relevant bladder and prostate cancer models. Importantly, the clonal architecture of the resultant tumors can be resolved using single-cell or spatially resolved next-generation sequencing to uncover polygenic drivers of cancer phenotypes.