학술논문
Yeast mitochondrial genomes consisting of only A.T base pairs replicate and exhibit suppressiveness.
Document Type
Article
Author
Source
Proceedings of the National Academy of Sciences of the United States of America; November 1984, Vol. 81 Issue: 22 p7156-7160, 5p
Subject
Language
ISSN
00278424; 10916490
Abstract
Mutants, called p-, that result from extensive deletions of the 75-kilobase Saccharomyces cerevisiae mitochondrial genome arise at high frequency. The remaining mitochondrial DNA is amplified in the p- cells, often as head-to-tail multimers, producing a cell with the normal amount of mitochondrial DNA. In matings, some of these p- mutants exhibit zygotic hypersuppressiveness, excluding the wild-type mitochondrial genome (p+) from all the diploids that are produced. From a hypersuppressive p- strain, we isolated two mutants with reduced suppressiveness. These mutants, one moderately suppressive and one nonsuppressive, retain only 89 and 70 base pairs, respectively, of the wild-type mitochondrial genome. Their sequences consist of 100% A . T base pairs. Replication of DNA in the mitochondrion, formation and amplification of new deletion genomes, and exhibition of suppressiveness do not require a single G . C base pair.