학술논문
A simple dynamic method for on-line and off-line determination of kLa during cultivation of animal cells
Document Type
Article
Author
Source
Biotechnology Techniques; September 1994, Vol. 8 Issue: 9 p675-680, 6p
Subject
Language
ISSN
0951208X; 15736784
Abstract
A new, fast method is described to determine kLa either off-line, or on-line during animal-cell cultivation. Since it does not need the equilibrium concentration of oxygen in the liquid phase (C*), it is not required to await a new steady state. Furthermore, the results do not depend on the calibration value of the dissolved-oxygen probe. The method yielded accurate values for kLa, both for an oxygen-consuming and a non-consuming system.