학술논문
Multiplex sequencing of 1.5 Mb of the Mycobacterium leprae genome.
Document Type
Article
Author
Smith, D R; Richterich, P; Rubenfield, M; Rice, P W; Butler, C; Lee, H M; Kirst, S; Gundersen, K; Abendschan, K; Xu, Q; Chung, M; Deloughery, C; Aldredge, T; Maher, J; Lundstrom, R; Tulig, C; Falls, K; Imrich, J; Torrey, D; Engelstein, M; Breton, G; Madan, D; Nietupski, R; Seitz, B; Connelly, S; McDougall, S; Safer, H; Gibson, R; Doucette-Stamm, L; Eiglmeier, K; Bergh, S; Cole, S T; Robison, K; Richterich, L; Johnson, J; Church, G M; Mao, J I
Source
Genome Research; August 1997, Vol. 7 Issue: 8 p802-819, 18p
Subject
Language
ISSN
10889051; 15495469
Abstract
The nucleotide sequence of 1.5 Mb of genomic DNA from Mycobacterium leprae was determined using computer-assisted multiplex sequencing technology. This brings the 2.8-Mb M. leprae genome sequence to approximately 66% completion. The sequences, derived from 43 recombinant cosmids, contain 1046 putative protein-coding genes, 44 repetitive regions, 3 tRNAs, and 15 tRNAs. The gene density of one per 1.4 kb is slightly lower than that of Mycoplasma (1.2 kb). Of the protein coding genes, 44% have significant matches to genes with well-defined functions. Comparison of 1157 M. leprae and 1564 Mycobacterium tuberculosis proteins shows a complex mosaic of homologous genomic blocks with up to 22 adjacent proteins in conserved map order. Matches to known enzymatic, antigenic, membrane, cell wall, cell division, multidrug resistance, and virulence proteins suggest therapeutic and vaccine targets. Unusual features of the M. leprae genome include large polyketide synthase (pks) operons, inteins, and highly fragmented pseudogenes.