학술논문
Identification of residues involved in binding of IL5 to βcomusing βIL3and βcomchimeras
Document Type
Article
Source
FEBS Letters; October 1999, Vol. 460 Issue: 1 p99-102, 4p
Subject
Language
ISSN
00145793
Abstract
In mice there are two forms of the beta chain used in the IL3 receptor system, βcomand βIL3. βcomis used by the IL3, IL5 and GM‐CSF receptors whereas βIL3is only used in the IL3 receptor. In this work an assay was developed to identify residues of βIL3that restrict IL5 activity. It was found that such residues reside within the 2nd CRM of the molecule. Furthermore, when residues in the βIL3B′‐C′ loop were replaced with βcomsequence a form of βIL3was produced that was able to respond to IL5. This region is also responsible for IL3 binding to βIL3in the absence of alpha chain. It is therefore an important structural motif of βcomand βIL3responsible for both ligand interaction and specificity.