학술논문
Hypothesis: naked plasmid DNA is taken up by cells in vivo by a receptor-mediated process
Document Type
Article
Author
Source
The Journal of Gene Medicine; March 2000, Vol. 2 Issue: 2 p76-88, 13p
Subject
Language
ISSN
1099498X; 15212254
Abstract
Following the initial demonstration that intramuscularly-injected naked plasmid DNA (pDNA) is expressed in myofibers, it was shown that pDNA can be used for vaccination purposes. More recent studies have indicated that naked pDNA can also achieve high levels of transgene expression in vivo. This efficiency of naked pDNA expression, especially via intravascular route, is truly astounding. In this prospective review, we examine the possible mechanisms of naked pDNA uptake. The possible mechanisms; (a) large membrane disruption, (b) small membrane pores, and (c) receptor-mediated endocytosis, are considered in turn. Some recent original laboratory data relevant to these hypotheses are also presented. Copyright © 2000 John Wiley & Sons, Ltd.