부산대학교 도서관

Experiments designed to analyze the lethality and hybridogenesis in the European green frog complex have yielded the following results: 1. As a rule the inter-se cross ofRana esculentais lethal, but several crosses have produced fully viable progeny. The frequency of such ‘break-through’ crosses appears to be related to parental population structure. 2. Parabiotic joining of lethal to viable embryos indicates that manifestation of the lethal effect is autonomous. There is, however, a 16–18% increase in the life span of the lethal partner. 3. Studies of LDH isozyme patterns revealed that thelessonae-specific alleles coding for the Baand Bcsubunits can be passed to the F1progeny from a parental female or male of theesculentaphenotype. This demonstrates that there is no total elimination of thelessonaegenome in theesculentagerm cells. 4. Immunologically, offspring from the inter-se cross ofR. esculentashow a closer relationship to theridibundathan to theesculentaphenotype. Variations of antigenic protein patterns suggest the possibility of chromosomal recombination betweenlessonaeandridibundain theesculentahybrid. These results are confirmed by two-dimensional electrophoretic analysis of proteins in the oocytes of the three frog phenotypes.