학술논문
Molekulargewichtsbestimmungen durch polyacrylamidgelelektrophorese unter verwendung eines linearen gelgradienten
Document Type
Article
Author
Source
FEBS Letters; November 1969, Vol. 5 Issue: 3 p221-224, 4p
Subject
Language
ISSN
00145793
Abstract
A new method for molecular weight determination using polyacrylamidegel electrophoresis in a linear gel‐concentration‐gradient (3–20°) is described. Plotting the log of molecular weights of several standard proteins against distance of migration or against 3 log of rate of n‐dgration reveal linear relationships in ranges of 50.000 – 200.000 or 100.000 – 400.000 Daltons respectively. On this basis, a simple method for molecular weight determination of proteins (accuracy ± 5°) has been devised. The method can also be applied for an individual protein in protein mixtures using specific staining procedures.