학술논문

Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations.
Document Type
Article
Source
Journal of Veterinary Medical Science; Oct2010, Vol. 72 Issue 10, p1283-1288, 6p, 2 Charts
Subject
BULLS
PARATUBERCULOSIS
MYCOBACTERIAL diseases in animals
POLYMERASE chain reaction
MYCOBACTERIUM avium
INTESTINES
LYMPH nodes
DISEASES
Language
ISSN
09167250
Abstract
This article discusses a study which examined the presence of bacteria in consecutive semen, blood and fecal samples from a Piedmont breeding bull with Johne's disease. The study used a quantitative real-time polymerase chain reaction (PCR) in the examination. It found five out of seven blood samples and all nine semen samples positive in the PCR. It cultured Mycobacterium avium subsp, paratubercldosis from various parts of the intestines, liver tissue and lymph nodes after the animal was euthanized.