학술논문
LINE-1 retrotransposons contribute to mouse PV interneuron development
Document Type
Article
Author
Bodea, Gabriela O.; Botto, Juan M.; Ferreiro, Maria E.; Sanchez-Luque, Francisco J.; de los Rios Barreda, Jose; Rasmussen, Jay; Rahman, Muhammed A.; Fenlon, Laura R.; Jansz, Natasha; Gubert, Carolina; Gerdes, Patricia; Bodea, Liviu-Gabriel; Ajjikuttira, Prabha; Da Costa Guevara, Darwin J.; Cumner, Linda; Bell, Charles C.; Kozulin, Peter; Billon, Victor; Morell, Santiago; Kempen, Marie-Jeanne H. C.; Love, Chloe J.; Saha, Karabi; Palmer, Lucy M.; Ewing, Adam D.; Jhaveri, Dhanisha J.; Richardson, Sandra R.; Hannan, Anthony J.; Faulkner, Geoffrey J.
Source
Nature Neuroscience; 20240101, Issue: Preprints p1-11, 11p
Subject
Language
ISSN
10976256; 15461726
Abstract
Retrotransposons are mobile DNA sequences duplicated via transcription and reverse transcription of an RNA intermediate. Cis-regulatory elements encoded by retrotransposons can also promote the transcription of adjacent genes. Somatic LINE-1 (L1) retrotransposon insertions have been detected in mammalian neurons. It is, however, unclear whether L1 sequences are mobile in only some neuronal lineages or therein promote neurodevelopmental gene expression. Here we report programmed L1 activation by SOX6, a transcription factor critical for parvalbumin (PV) interneuron development. Mouse PV interneurons permit L1 mobilization in vitro and in vivo, harbor unmethylated L1 promoters and express full-length L1 mRNAs and proteins. Using nanopore long-read sequencing, we identify unmethylated L1s proximal to PV interneuron genes, including a novel L1 promoter-driven Caps2transcript isoform that enhances neuron morphological complexity in vitro. These data highlight the contribution made by L1 cis-regulatory elements to PV interneuron development and transcriptome diversity, uncovered due to L1 mobility in this milieu.