학술논문
Characterization of lactose transport in Kluyveromyces lactis
Document Type
Article
Author
Source
Journal of Bacteriology; June 1983, Vol. 154 Issue: 3 p1245-1251, 7p
Subject
Language
ISSN
00219193; 10985530
Abstract
We have determined that lactose uptake in Kluyveromyces lactis is mediated by an inducible transport system. Induction, elicited by lactose or galactose, of the transporter required protein synthesis. Transport of lactose required an energy-generating system and occurred by an active process, since an intracellular lactose concentration 175 times greater than the extracellular concentration could be obtained. The Km for lactose transport was about 2.8 mM in uninduced and lactose- or galactose-induced cells. The lactose transporters in K. lactis and Escherichia coli appear to be different since they respond uniquely to inhibition by substrate analogs.