학술논문
Rapid Sequencing of the Mycobacterium tuberculosispncAGene for Detection of Pyrazinamide Susceptibility
Document Type
Article
Author
Source
Journal of Clinical Microbiology; November 2014, Vol. 52 Issue: 11 p4056-4057, 2p
Subject
Language
ISSN
00951137; 1098660X
Abstract
ABSTRACTWe developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to pyrazinamide.