학술논문
Population genomic analysis of Aegilops tauschiiidentifies targets for bread wheat improvement
Document Type
Article
Author
Gaurav, Kumar; Arora, Sanu; Silva, Paula; Sánchez-Martín, Javier; Horsnell, Richard; Gao, Liangliang; Brar, Gurcharn S.; Widrig, Victoria; John Raupp, W.; Singh, Narinder; Wu, Shuangye; Kale, Sandip M.; Chinoy, Catherine; Nicholson, Paul; Quiroz-Chávez, Jesús; Simmonds, James; Hayta, Sadiye; Smedley, Mark A.; Harwood, Wendy; Pearce, Suzannah; Gilbert, David; Kangara, Ngonidzashe; Gardener, Catherine; Forner-Martínez, Macarena; Liu, Jiaqian; Yu, Guotai; Boden, Scott A.; Pascucci, Attilio; Ghosh, Sreya; Hafeez, Amber N.; O’Hara, Tom; Waites, Joshua; Cheema, Jitender; Steuernagel, Burkhard; Patpour, Mehran; Justesen, Annemarie Fejer; Liu, Shuyu; Rudd, Jackie C.; Avni, Raz; Sharon, Amir; Steiner, Barbara; Kirana, Rizky Pasthika; Buerstmayr, Hermann; Mehrabi, Ali A.; Nasyrova, Firuza Y.; Chayut, Noam; Matny, Oadi; Steffenson, Brian J.; Sandhu, Nitika; Chhuneja, Parveen; Lagudah, Evans; Elkot, Ahmed F.; Tyrrell, Simon; Bian, Xingdong; Davey, Robert P.; Simonsen, Martin; Schauser, Leif; Tiwari, Vijay K.; Randy Kutcher, H.; Hucl, Pierre; Li, Aili; Liu, Deng-Cai; Mao, Long; Xu, Steven; Brown-Guedira, Gina; Faris, Justin; Dvorak, Jan; Luo, Ming-Cheng; Krasileva, Ksenia; Lux, Thomas; Artmeier, Susanne; Mayer, Klaus F. X.; Uauy, Cristobal; Mascher, Martin; Bentley, Alison R.; Keller, Beat; Poland, Jesse; Wulff, Brande B. H.
Source
Nature Biotechnology; March 2022, Vol. 40 Issue: 3 p422-431, 10p
Subject
Language
ISSN
10870156; 15461696
Abstract
Aegilops tauschii, the diploid wild progenitor of the D subgenome of bread wheat, is a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. Here we sequenced 242 Ae. tauschiiaccessions and compared them to the wheat D subgenome to characterize genomic diversity. We found that a rare lineage of Ae. tauschiigeographically restricted to present-day Georgia contributed to the wheat D subgenome in the independent hybridizations that gave rise to modern bread wheat. Through k-mer-based association mapping, we identified discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of hexaploids incorporating diverse Ae. tauschiigenomes. Exploiting the genomic diversity of the Ae. tauschiiancestral diploid genome permits rapid trait discovery and functional genetic validation in a hexaploid background amenable to breeding.