부산대학교 도서관

Various agro-industrial wastes are utilized as fermentation raw materials and are considered promising biorefinery substrates. The search for novel substrates is an ongoing quest, particularly for those residues that do not interfere with the food chain and do not need arable land. Here, a halophytic biomass from Arthrocnemum macrostachyumwas utilized as a substrate to produce multienzyme preparation from two endophytic strains of Bacillussp. TKE1 and TKE4. Initially, a one-factor-at-a-time approach was adopted to investigate the appropriate levels of the variables affecting enzyme production. Consequently, the central composite design was used to optimize the enzymes produced by the two strains. Among the enzymes studied, amylase and xylanase activity were found to have significant R2values (> 90%) in ANOVA; hence, these were further optimized. Response surface optimization of the enzymes production resulted in the final titers of 4.3 IU mL−1of amylase and 3.5 IU mL−1of xylanase by TKE1 while 3.2 IU mL−1of amylase and 2.9 IU mL−1of xylanase by TKE4. The SEM analysis revealed the separation of substrate fibers, loosening of the matrix, porous, and increased contact surface. The FTIR results showed significant changes in the lignin-related bands (1518 to 1257 cm−1), changes in the bands for cellulosic component (1635.63 cm−1and 1608.63 cm−1), starch content (1064.70 cm−1), and β-glycosidic linkage (848.67 and 864.10 cm−1), indicating the degradation of the substrate by the strains. The study concludes that the halophytic biomass from A. macrostachyumcan serve as a substrate for industrial enzyme production.