학술논문
The use of dried blood spots for the serological evaluation of SARS-CoV-2 antibodies.
Document Type
Academic Journal
Author
Toh ZQ; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.; Higgins RA; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Anderson J; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.; Mazarakis N; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Victoria 3052, Australia.; Do LAH; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.; Rautenbacher K; Department of General Medicine, The Royal Children's Hospital, Parkville, Victoria 3052, Australia.; Ramos P; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Dohle K; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Tosif S; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.; Department of General Medicine, The Royal Children's Hospital, Parkville, Victoria 3052, Australia.; Crawford N; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.; Department of General Medicine, The Royal Children's Hospital, Parkville, Victoria 3052, Australia.; Mulholland K; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.; Department of Infectious Disease and Epidemiology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK.; Licciardi PV; Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.
Source
Publisher: Oxford University Press Country of Publication: England NLM ID: 101188638 Publication Model: Print Cited Medium: Internet ISSN: 1741-3850 (Electronic) Linking ISSN: 17413842 NLM ISO Abbreviation: J Public Health (Oxf) Subsets: MEDLINE
Subject
Language
English
Abstract
Background: To determine if dried blood spot specimens (DBS) can reliably detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies, we compared the SARS-CoV-2 IgG antibody response in paired serum and eluates from DBS specimens.
Methods: A total of 95 paired DBS and serum samples were collected from 74 participants (aged 1-63 years) as part of a household cohort study in Melbourne, Australia. SARS-CoV-2 IgG antibodies specific for the receptor-binding domain (RBD) and S1 proteins between serum and eluates from DBS specimens were compared using an FDA-approved ELISA method.
Results: Among the 74 participants, 42% (31/74) were children and the rest were adults. A total of 16 children and 13 adults were SARS-CoV-2 positive by polymerase chain reaction. The IgG seropositivity rate was similar between serum and DBS specimens (18.9% (18/95) versus 16.8% (16/95)), respectively. Similar RBD and S1-specific IgG levels were detected between serum and DBS specimens. Serum IgG levels strongly correlated with DBS IgG levels (r = 0.99, P < 0.0001) for both SARS-CoV-2 proteins. Furthermore, antibodies remained stable in DBS specimens for >3 months.
Conclusions: DBS specimens can be reliably used as an alternative to serum samples for SARS-CoV-2 antibody measurement. The use of DBS specimens would facilitate serosurveillance efforts particularly in hard-to-reach populations and inform public health responses including COVID-19 vaccination strategies.
(© The Author(s) 2021. Published by Oxford University Press on behalf of Faculty of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Methods: A total of 95 paired DBS and serum samples were collected from 74 participants (aged 1-63 years) as part of a household cohort study in Melbourne, Australia. SARS-CoV-2 IgG antibodies specific for the receptor-binding domain (RBD) and S1 proteins between serum and eluates from DBS specimens were compared using an FDA-approved ELISA method.
Results: Among the 74 participants, 42% (31/74) were children and the rest were adults. A total of 16 children and 13 adults were SARS-CoV-2 positive by polymerase chain reaction. The IgG seropositivity rate was similar between serum and DBS specimens (18.9% (18/95) versus 16.8% (16/95)), respectively. Similar RBD and S1-specific IgG levels were detected between serum and DBS specimens. Serum IgG levels strongly correlated with DBS IgG levels (r = 0.99, P < 0.0001) for both SARS-CoV-2 proteins. Furthermore, antibodies remained stable in DBS specimens for >3 months.
Conclusions: DBS specimens can be reliably used as an alternative to serum samples for SARS-CoV-2 antibody measurement. The use of DBS specimens would facilitate serosurveillance efforts particularly in hard-to-reach populations and inform public health responses including COVID-19 vaccination strategies.
(© The Author(s) 2021. Published by Oxford University Press on behalf of Faculty of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)