학술논문
Bimodal modulation of in vitro angiogenesis with photoactive polymer nanoparticles.
Document Type
Academic Journal
Author
Tullii G; Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, 20134 Milano, Italy. gabriele.tullii@iit.it.; Gutierrez-Fernandez E; POLYMAT, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain.; Ronchi C; Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, 20134 Milano, Italy. gabriele.tullii@iit.it.; Bellacanzone C; Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, 20134 Milano, Italy. gabriele.tullii@iit.it.; Bondi L; DiFA University of Bologna, Viale Carlo Berti Pichat 6/2 Bologna, 40127, Italy.; Criado-Gonzalez M; POLYMAT, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain.; Lagonegro P; Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, 20134 Milano, Italy. gabriele.tullii@iit.it.; Moccia F; Department of Biology and Biotechnology 'Lazzaro Spallanzani', University of Pavia, 27100 Pavia, Italy.; Cramer T; DiFA University of Bologna, Viale Carlo Berti Pichat 6/2 Bologna, 40127, Italy.; Mecerreyes D; POLYMAT, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain.; Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain.; Martín J; POLYMAT, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain.; Universidade da Coruña, Campus Industrial de Ferrol, CITENI, Campus Esteiro S/N, 15403 Ferrol, Spain.; Antognazza MR; Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, 20134 Milano, Italy. gabriele.tullii@iit.it.
Source
Publisher: RSC Pub Country of Publication: England NLM ID: 101525249 Publication Model: Electronic Cited Medium: Internet ISSN: 2040-3372 (Electronic) Linking ISSN: 20403364 NLM ISO Abbreviation: Nanoscale Subsets: MEDLINE
Subject
Language
English
Abstract
Angiogenesis is a fundamental process in biology, given the pivotal role played by blood vessels in providing oxygen and nutrients to tissues, thus ensuring cell survival. Moreover, it is critical in many life-threatening pathologies, like cancer and cardiovascular diseases. In this context, conventional treatments of pathological angiogenesis suffer from several limitations, including low bioavailability, limited spatial and temporal resolution, lack of specificity and possible side effects. Recently, innovative strategies have been explored to overcome these drawbacks based on the use of exogenous nano-sized materials and the treatment of the endothelial tissue with optical or electrical stimuli. Here, conjugated polymer-based nanoparticles are proposed as exogenous photo-actuators, thus combining the advantages offered by nanotechnology with those typical of optical stimulation. Light excitation can achieve high spatial and temporal resolution, while permitting minimal invasiveness. Interestingly, the possibility to either enhance (≈+30%) or reduce (up to -65%) the angiogenic capability of model endothelial cells is demonstrated, by employing different polymer beads, depending on the material type and the presence/absence of the light stimulus. In vitro results reported here represent a valuable proof of principle of the reliability and efficacy of the proposed approach and should be considered as a promising step towards a paradigm shift in therapeutic angiogenesis.