학술논문
Synergistic interaction of CLAVATA1, CLAVATA2, and RECEPTOR-LIKE PROTEIN KINASE 2 in cyst nematode parasitism of Arabidopsis.
Document Type
Academic Journal
Author
Replogle A; University of Missouri, Columbia, MO, USA.; Wang J; Paolillo V; Smeda J; Kinoshita A; Durbak A; Tax FE; Wang X; Sawa S; Mitchum MG
Source
Publisher: APS Press Country of Publication: United States NLM ID: 9107902 Publication Model: Print Cited Medium: Print ISSN: 0894-0282 (Print) Linking ISSN: 08940282 NLM ISO Abbreviation: Mol Plant Microbe Interact Subsets: MEDLINE
Subject
Language
English
ISSN
0894-0282
Abstract
Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ENDOSPERM SURROUNDING REGION (CLE)-like effector proteins. These proteins act as ligand mimics of plant CLE peptides and are required for successful nematode infection. Previously, we showed that the CLV2/CORYNE (CRN) heterodimer receptor complex is required for nematode CLE signaling. However, there was only a partial reduction in nematode infection when this signaling was disrupted, indicating that there might be additional nematode CLE receptors. In this study, we demonstrate that CLV1 and RECEPTOR-LIKE PROTEIN KINASE 2/TOADSTOOL2 (RPK2), two additional receptors that can transmit the CLV3 signal independent of CLV2/CRN for shoot apical meristem maintenance, also play a role in nematode CLE perception. Localization studies showed that both receptors are expressed in nematode-induced syncytia. Infection assays with clv1 and rpk2 single mutants revealed a decrease in both nematode infection and syncytium size. Significantly, further reduction in nematode infection was observed when rpk2 was combined with clv1 and clv2 mutants. Taken together, our results indicate that parallel signaling pathways involving CLV1, CLV2, and RPK2 are important for nematode parasitism.