학술논문
Periosteum-induced ossification effect in skull defect through interleukin-8 and NF-κB pathway: An experimental study with Oryctolagus cuniculus rabbits.
Document Type
Academic Journal
Author
Apriawan T; Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital.; Widjiati W; Faculty of Veterinary Medicine, Universitas Airlangga.; Utomo DN; Departments of Orthopaedics and Traumatology, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital, Surabaya.; Fauzi AA; Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital.; Subagio EA; Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital.; Utomo B; Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital, Surabaya.; Islam AA; Department of Neurosurgery, Faculty of Medicine, Universitas Hasanuddin - Dr. Wahidin Sudirohusodo Central General Hospital, Makassar, South Celebes.; Bajamal AH; Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital.; Sudiana IK; Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital, Surabaya, East Java, Indonesia.
Source
Publisher: Scientific Scholar LLC Country of Publication: United States NLM ID: 101535836 Publication Model: eCollection Cited Medium: Print ISSN: 2229-5097 (Print) Linking ISSN: 21527806 NLM ISO Abbreviation: Surg Neurol Int Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2229-5097
Abstract
Background: The purpose of this study was to analyze the response of inflammatory cytokines interleukin-8 (IL-8) and NF-κB to the closure of skull defect with periosteum as a scaffolding material in bone healing used after surgery.
Methods: Thirty Oryctolagus cuniculus rabbits underwent a craniotomy to create a 20 mm diameter round defect in the parietal bones. The parietal bones were returned to its place and stabilized by an internal plate fixation. The defects were either left empty or implanted with periosteum. At 6 weeks, the specimens were euthanized and examined.
Results: Histological examination showed a more well-developed formation of woven bone in the periosteum group. Immunohistochemical examinations showed that the use of periosteum in the closure of skull defects reduced the NF-κB and IL-8 response which affected the ossification process.
Conclusion: The experiment showed that the use of periosteum was linked with IL-8 and NF-κB downregulation toward ossification effects at any point throughout the trial. Periosteum usage might be beneficial as a scaffolding material in bone healing for autograft cranioplasty in animal model and could be applied to clinical practice.
Competing Interests: There are no conflicts of interest.
(Copyright: © 2022 Surgical Neurology International.)
Methods: Thirty Oryctolagus cuniculus rabbits underwent a craniotomy to create a 20 mm diameter round defect in the parietal bones. The parietal bones were returned to its place and stabilized by an internal plate fixation. The defects were either left empty or implanted with periosteum. At 6 weeks, the specimens were euthanized and examined.
Results: Histological examination showed a more well-developed formation of woven bone in the periosteum group. Immunohistochemical examinations showed that the use of periosteum in the closure of skull defects reduced the NF-κB and IL-8 response which affected the ossification process.
Conclusion: The experiment showed that the use of periosteum was linked with IL-8 and NF-κB downregulation toward ossification effects at any point throughout the trial. Periosteum usage might be beneficial as a scaffolding material in bone healing for autograft cranioplasty in animal model and could be applied to clinical practice.
Competing Interests: There are no conflicts of interest.
(Copyright: © 2022 Surgical Neurology International.)