학술논문
Phosphoproteomics--more than meets the eye.
Document Type
Academic Journal
Author
Loroch S; Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V, Dortmund, Germany.; Dickhut C; Zahedi RP; Sickmann A
Source
Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE
Subject
Language
English
Abstract
PTMs enable cells to adapt to internal and external stimuli in the milliseconds to seconds time regime. Protein phosphorylation is probably the most important of these modifications as it affects protein structure and interactions, critically influencing the life cycle of a cell. In the last 15 years, new insights into phosphorylation have been provided by highly sensitive MS-based approaches combined with specific phosphopeptide enrichment strategies. Although so far research has mainly focused on the discovery and characterization of O-phosphorylation, this review also briefly outlines the current knowledge about N-phosphorylation depicting its ubiquitous relevance. Further, common pitfalls in sample preparation, LC-MS analysis, and subsequent data analysis are discussed as well as issues regarding quality and comparability of studies on protein phosphorylation.
(© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
(© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)