학술논문
Optical Magnetometry of Single Biocompatible Micromagnets for Quantitative Magnetogenetic and Magnetomechanical Assays.
Document Type
Academic Journal
Author
Toraille L; Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Université Paris-Saclay , 91405 Orsay , France.; Aïzel K; Laboratoire Physico-Chimie , Institut Curie, CNRS UMR168, PSL Research University , Université Pierre et Marie Curie-Paris 6 , 75248 Paris Cedex 05, France.; Balloul É; Laboratoire Physico-Chimie , Institut Curie, CNRS UMR168, PSL Research University , Université Pierre et Marie Curie-Paris 6 , 75248 Paris Cedex 05, France.; Vicario C; Laboratoire Physico-Chimie , Institut Curie, CNRS UMR168, PSL Research University , Université Pierre et Marie Curie-Paris 6 , 75248 Paris Cedex 05, France.; Monzel C; Laboratoire Physico-Chimie , Institut Curie, CNRS UMR168, PSL Research University , Université Pierre et Marie Curie-Paris 6 , 75248 Paris Cedex 05, France.; Experimental Medical Physics , Heinrich-Heine University Düsseldorf , Universitätsstrasse 1 , 40225 Düsseldorf , Germany.; Coppey M; Laboratoire Physico-Chimie , Institut Curie, CNRS UMR168, PSL Research University , Université Pierre et Marie Curie-Paris 6 , 75248 Paris Cedex 05, France.; Secret E; Physico-chimie des électrolytes et nanosystèmes interfaciaux , PHENIX, CNRS UMR 8234, Sorbonne Université , F-75005 Paris , France.; Siaugue JM; Physico-chimie des électrolytes et nanosystèmes interfaciaux , PHENIX, CNRS UMR 8234, Sorbonne Université , F-75005 Paris , France.; Sampaio J; Laboratoire de Physique des Solides, CNRS, Université Paris-Sud, Université Paris-Saclay , 91405 Orsay , France.; Rohart S; Laboratoire de Physique des Solides, CNRS, Université Paris-Sud, Université Paris-Saclay , 91405 Orsay , France.; Vernier N; Centre de Nanosciences et de Nanotechnologies , CNRS, Université Paris-Sud, Université Paris-Saclay , 91405 Orsay , France.; Bonnemay L; Alveole , 30 rue de Campo Formio , 75013 Paris , France.; Debuisschert T; Thales Research & Technology , 1 avenue Augustin Fresnel , 91767 Palaiseau Cedex , France.; Rondin L; Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Université Paris-Saclay , 91405 Orsay , France.; Roch JF; Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Université Paris-Saclay , 91405 Orsay , France.; Dahan M; Laboratoire Physico-Chimie , Institut Curie, CNRS UMR168, PSL Research University , Université Pierre et Marie Curie-Paris 6 , 75248 Paris Cedex 05, France.
Source
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101088070 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6992 (Electronic) Linking ISSN: 15306984 NLM ISO Abbreviation: Nano Lett Subsets: MEDLINE
Subject
Language
English
Abstract
The mechanical manipulation of magnetic nanoparticles is a powerful approach to probing and actuating biological processes in living systems. Implementing this technique in high-throughput assays can be achieved using biocompatible micromagnet arrays. However, the magnetic properties of these arrays are usually indirectly inferred from simulations or Stokes drag measurements, leaving unresolved questions about the actual profile of the magnetic fields at the micrometer scale and the exact magnetic forces that are applied. Here, we exploit the magnetic field sensitivity of nitrogen-vacancy color centers in diamond to map the 3D stray magnetic field produced by a single soft ferromagnetic microstructure. By combining this wide-field optical magnetometry technique with magneto-optic Kerr effect microscopy, we fully analyze the properties of the micromagnets, including their magnetization saturation and their size-dependent magnetic susceptibility. We further show that the high magnetic field gradients produced by the micromagnets, greater than 10 4 T·m -1 under an applied magnetic field of about 100 mT, enables the manipulation of magnetic nanoparticles smaller than 10 nm inside living cells. This work paves the way for quantitative and parallelized experiments in magnetogenetics and magnetomechanics in cell biology.