학술논문
Association of macro-and micro-nutrients dietary intakes with rs2241883 genetic variants of FABP 1 gene in MASHAD study population.
Document Type
Academic Journal
Author
Valizadeh M; Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Aghasizadeh M; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.; Saberi-Karimian M; Endoscopic and Minimally Invasive Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.; Safari M; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.; Rohban M; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.; Bana HS; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.; Zare-Feyzabadi R; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.; Tavakkol Afshari HS; Department of Biology, Ashkezar Branch, Islamic Azad University, Mashhad, Iran.; Moradi A; Department of Biology, Ashkezar Branch, Islamic Azad University, Mashhad, Iran.; Ahangari N; Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Hashemi M; Department of Clinical Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Nematy M; Department of Clinical Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Bahre EA; Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.; Aghaei-Bakhtiari SH; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.; Ghazizadeh H; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.; Esmaily H; Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.; Ferns GA; Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex, BN1 9PH, UK.; Pasdar A; Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Bioinformatics Research Group, Mashhad University of Medical Sciences, Mashhad, Iran; Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen, UK. Electronic address: pasdara@mums.ac.ir.; Ghayour-Mobarhan M; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.
Source
Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101654592 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-4577 (Electronic) Linking ISSN: 24054577 NLM ISO Abbreviation: Clin Nutr ESPEN Subsets: MEDLINE
Subject
Language
English
Abstract
Introduction: There is a relationship between macro-nutrient-intakes and the genes implicated in lipid metabolism. In this study, we assessed the association between macro-and micro-nutrients dietary intakes with rs2241883 genetic variants of the FABP1 gene.
Methods: For this cross-sectional study 2737 subjects (including 2203 subjects with dyslipidemia and 534 healthy volunteers) were enrolled as part of the Mashhad Stroke and Heart Atherosclerotic Disorder (MASHAD) study cohort. Dyslipidemia was defined based on the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III). A NanoDrop®-1000 instrument was used to do the quantitation of DNA. The rs2241883 polymorphisms were genotyped using double ARMs PCR reactions. Genotyping reagents were obtained from Applied Biosystems. Dietary intake was evaluated using a food frequency questionnaire (FFQ) and validated by 2 consecutive 24-h food recalls.
Results: The results showed no significant association between subjects with and without dyslipidemia (P > 0.05), except for the zinc to copper ratio, the value for which was higher in the subjects with dyslipidemia (4.78 (1.62)) when compared to subjects without dyslipidemia (4.68 (1.82)) (p = 0.05). Using different genetic models we found that zinc and copper were significantly different in the additive (p = 0.01) and dominant (p = 0.01) genetic models. Although, this association was no longer significant after adjusting for confounding factors.
Conclusions: There were no associations between macro-and micro-nutrient dietary intakes with rs2241883 genetic variants after adjusting for confounding factors in the MASHAD study population.
Competing Interests: Declaration of competing interest The authors confirm no conflict of interest.
(Copyright © 2021. Published by Elsevier Ltd.)
Methods: For this cross-sectional study 2737 subjects (including 2203 subjects with dyslipidemia and 534 healthy volunteers) were enrolled as part of the Mashhad Stroke and Heart Atherosclerotic Disorder (MASHAD) study cohort. Dyslipidemia was defined based on the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III). A NanoDrop®-1000 instrument was used to do the quantitation of DNA. The rs2241883 polymorphisms were genotyped using double ARMs PCR reactions. Genotyping reagents were obtained from Applied Biosystems. Dietary intake was evaluated using a food frequency questionnaire (FFQ) and validated by 2 consecutive 24-h food recalls.
Results: The results showed no significant association between subjects with and without dyslipidemia (P > 0.05), except for the zinc to copper ratio, the value for which was higher in the subjects with dyslipidemia (4.78 (1.62)) when compared to subjects without dyslipidemia (4.68 (1.82)) (p = 0.05). Using different genetic models we found that zinc and copper were significantly different in the additive (p = 0.01) and dominant (p = 0.01) genetic models. Although, this association was no longer significant after adjusting for confounding factors.
Conclusions: There were no associations between macro-and micro-nutrient dietary intakes with rs2241883 genetic variants after adjusting for confounding factors in the MASHAD study population.
Competing Interests: Declaration of competing interest The authors confirm no conflict of interest.
(Copyright © 2021. Published by Elsevier Ltd.)