학술논문
Understanding PFAS toxicity through cell culture metabolomics: Current applications and future perspectives.
Document Type
Academic Journal
Author
Nguyen TV; Environment, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Waite Campus, South Australia 5064, Australia; NTT Institute of High Technology, Nguyen Tat Thanh University, 300A Nguyen Tat Thanh, District 4, Ho Chi Minh City, Viet Nam. Electronic address: thomas.nguyen@csiro.au.; Trang PN; Department of Food Technology, Institute of Food and Biotechnology, Can Tho University, Campus II, 3/2 Street, Ninh Kieu District, Can Tho, Viet Nam. Electronic address: pntrang@ctu.edu.vn.; Kumar A; Environment, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Waite Campus, South Australia 5064, Australia. Electronic address: Anupama.Kumar@csiro.au.
Source
Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 7807270 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6750 (Electronic) Linking ISSN: 01604120 NLM ISO Abbreviation: Environ Int Subsets: MEDLINE
Subject
Language
English
Abstract
Per- and polyfluoroalkyl substances (PFAS), ubiquitous environmental contaminants, pose significant challenges to ecosystems and human health. While cell cultures have emerged as new approach methodologies (NAMs) in ecotoxicity research, metabolomics is an emerging technique used to characterize the small-molecule metabolites present in cells and to understand their role in various biological processes. Integration of metabolomics with cell cultures, known as cell culture metabolomics, provides a novel and robust tool to unravel the complex molecular responses induced by PFAS exposure. In vitro testing also reduces reliance on animal testing, aligning with ethical and regulatory imperatives. The current review summarizes key findings from recent studies utilizing cell culture metabolomics to investigate PFAS toxicity, highlighting alterations in metabolic pathways, biomarker identification, and the potential linkages between metabolic perturbations. Additionally, the paper discusses different types of cell cultures and metabolomics methods used for studies of environmental contaminants and particularly PFAS. Future perspectives on the combination of metabolomics with other advanced technologies, such as single-cell metabolomics (SCM), imaging mass spectrometry (IMS), extracellular flux analysis (EFA), and multi-omics are also explored, which offers a holistic understanding of environmental contaminants. The synthesis of current knowledge and identification of research gaps provide a foundation for future investigations that aim to elucidate the complexities of PFAS-induced cellular responses and contribute to the development of effective strategies for mitigating their adverse effects on human health.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)