학술논문
LncRNA RP1-276N6.2 Expression and RP1-276N6.2 Gene Polymorphisms Contribute to the Risk of Coronary Artery Disease in Chinese Han Population.
Document Type
Academic Journal
Author
Chen L; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Zhao M; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Zhou M; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Luo J; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Li S; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Liu X; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Cheng Z; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Zhuo Y; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Zeng W; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Zhang Z; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.; Zhou L; Department of Epidemiology, School of Public Health, Chongqing Medical University, Chongqing, China.
Source
Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 9004522 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7430 (Electronic) Linking ISSN: 10445498 NLM ISO Abbreviation: DNA Cell Biol Subsets: MEDLINE
Subject
Language
English
Abstract
Long noncoding RNAs (lncRNAs) have been implicated in coronary artery disease (CAD) processes. However, the relationship between the gene polymorphisms of lncRNA RP1-276N6.2 as a novel molecule and susceptibility to CAD remains unclear. In our case-control study, 949 CAD patients and 892 healthy controls were genotyped using the TaqMan genotyping assay. The quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay were performed to examine the expression levels of RP1-276N6.2 and SLC22A3(OCT3). We observed that CAD patients had significantly lower RP1-276N6.2 levels than those healthy participants ( p < 0.05). Compared to the wild-type genotype, the rs611950 T allele and the rs10499313 AG genotype and G allele significantly increased the premature CAD risk ( p = 0.02, p = 0.002, and p = 0.01, respectively), while the rs505000 G allele reduced this risk ( p = 0.01); moreover, the rs505000 CG genotype significantly enhanced the delayed CAD risk ( p = 0.03), and the rs505000 G allele reduced the expression levels of RP1-276N6.2 and SLC22A3 ( p < 0.05 and p < 0.05, respectively). In addition, RP1-276N6.2 positively regulated the mRNA and secreted protein levels of SLC22A3 ( p < 0.05). In conclusion, the RP1-276N6.2 gene polymorphisms were closely associated with CAD risk. LncRNA RP1-276N6.2 may be a potential genetic target for CAD early diagnosis and treatment.