학술논문
Effect of an Enteroprotective Complementary Feed on Faecal Markers of Inflammation and Intestinal Microbiota Composition in Weaning Puppies.
Document Type
Academic Journal
Author
Meineri G; Department of Veterinary Sciences, School of Agriculture and Veterinary Medicine, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.; Cocolin L; Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.; Morelli G; CeDIS (Science Information and Documentation Center), Innovet Italia Srl, Via Leonardo Da Vinci 3, 35030 Saccolongo, Italy.; Schievano C; Innovative Statistical Research Srl, Prato della Valle 24, 35123 Padova, Italy.; Atuahene D; Department of Veterinary Sciences, School of Agriculture and Veterinary Medicine, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.; Ferrocino I; Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.
Source
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101680127 Publication Model: Electronic Cited Medium: Internet ISSN: 2306-7381 (Electronic) Linking ISSN: 23067381 NLM ISO Abbreviation: Vet Sci Subsets: PubMed not MEDLINE
Subject
Language
English
Abstract
Weaning entails numerous modifications of the intestinal structure and microbiota composition, making puppies at high risk of sickness during this delicate life stage. The aim of this study was to investigate the effects of a four-week administration of a supplement composed of ultramicronised Palmitoylethanolamide, bovine colostrum and Bacillus subtilis (Normalia ® Extra, Innovet Italia Srl, Saccolongo, Italy) on markers of gut health and microbiome of weaning puppies. Twenty-nine four-week-old Golden Retriever puppies were randomly assigned to control (CG, n = 13) and treated (TG, n = 16) groups. During the whole experimental time, there were no differences between the groups with regard to average daily gain and faecal score. In TG, faecal calprotectin and zonulin values were statistically significantly decreased compared to CG, especially at week 8 (zonulin: 42.8 ± 1.54 ng/mL and 55.3 ± 42.8 ng/mL, and calprotectin: 2.91 ± 0.38 µg/g and 5.71 ± 0.43 µg/g, in TG and CG, respectively; p < 0.0001 for both comparisons). Bacteria belonging to phylum Campylobacterota decreased ( p = 0.04), while those referring to genera Coprococcus and Pseudomonas increased ( p = 0.01 and p = 0.04, respectively). The supplementation of the tested complementary feed can promote the intestinal health of puppies and therefore facilitate weaning by lowering gut inflammation.