학술논문
A minimally destructive protocol for DNA extraction from ancient teeth.
Document Type
Academic Journal
Author
Harney É; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.; The Max Planck-Harvard Research Center for the Archaeoscience of the Ancient Mediterranean, Cambridge, Massachusetts 02138, USA and Jena D-07745, Germany.; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Department of Human Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.; Cheronet O; Department of Evolutionary Anthropology, University of Vienna, Vienna 1090, Austria.; Fernandes DM; Department of Evolutionary Anthropology, University of Vienna, Vienna 1090, Austria.; CIAS, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal.; Sirak K; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Department of Human Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.; Mah M; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Bernardos R; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Adamski N; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Broomandkhoshbacht N; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Callan K; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Lawson AM; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Oppenheimer J; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Stewardson K; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Zalzala F; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Anders A; Institute of Archaeological Sciences, Eötvös Loránd University, 1088 Budapest, Hungary.; Candilio F; Superintendency of Archaeology, Fine Arts and Landscape for the City of Cagliari and the Provinces of Oristano and South Sardinia, 09121 Cagliari, Italy.; Constantinescu M; Fr. I. Rainer Institute of Anthropology, Bucharest 050711, Romania.; Coppa A; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Department of Evolutionary Anthropology, University of Vienna, Vienna 1090, Austria.; Department of Environmental Biology, Sapienza University, 00185 Rome, Italy.; Ciobanu I; Cultural-Natural Reserve 'Orheiul Vechi', 3552 Orhei, Republic of Moldova.; Institute of Bioarchaeological and Ethnocultural Research, 2012 Chișinău, Republic of Moldova.; Dani J; Déri Museum, 4026 Debrecen, Hungary.; Gallina Z; Ásatárs Kulturális, Régészeti Szolgáltató és Kereskedelmi Limited, 6000 Kecskemét, Hungary.; Genchi F; Department of Environmental Biology, Sapienza University, 00185 Rome, Italy.; Nagy EG; Déri Museum, 4026 Debrecen, Hungary.; Hajdu T; Department of Biological Anthropology, Eötvös Loránd University, 1171 Budapest, Hungary.; Department of Anthropology, Hungarian Natural History Museum, 1083 Budapest, Hungary.; Hellebrandt M; Herman Ottó Museum, 3529 Miskolc, Hungary.; Horváth A; Herman Ottó Museum, 3529 Miskolc, Hungary.; Király Á; Institute of Archaeology, Research Centre for the Humanities, 1097 Budapest, Hungary.; Kiss K; Department of Biological Anthropology, Eötvös Loránd University, 1171 Budapest, Hungary.; Department of Anthropology, Hungarian Natural History Museum, 1083 Budapest, Hungary.; Kolozsi B; Déri Museum, 4026 Debrecen, Hungary.; Kovács P; Damjanich János Museum, 5000 Szolnok, Hungary.; Köhler K; Institute of Archaeology, Research Centre for the Humanities, 1097 Budapest, Hungary.; Lucci M; Department of History, Anthropology, Religion, Arts and Performing Arts, Sapienza University, 00185 Rome, Italy.; Pap I; Department of Anthropology, Hungarian Natural History Museum, 1083 Budapest, Hungary.; Popovici S; National Agency for Archaeology, 2012 Chișinău, Republic of Moldova.; Raczky P; Institute of Archaeological Sciences, Eötvös Loránd University, 1088 Budapest, Hungary.; Simalcsik A; Institute of Bioarchaeological and Ethnocultural Research, 2012 Chișinău, Republic of Moldova.; Olga Necrasov Center for Anthropological Research, Romanian Academy, 700481 Iasi, Romania.; Szeniczey T; Department of Biological Anthropology, Eötvös Loránd University, 1171 Budapest, Hungary.; Department of Anthropology, Hungarian Natural History Museum, 1083 Budapest, Hungary.; Vasilyev S; Institute of Ethnology and Anthropology of the Russian Academy of Sciences, 119991 Moscow, Russia.; Center for Egyptological Studies of the Russian Academy of Sciences, 119071 Moscow, Russia.; Virag C; Satu Mare County Museum, 440031 Satu Mare, Romania.; Rohland N; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA.; Reich D; The Max Planck-Harvard Research Center for the Archaeoscience of the Ancient Mediterranean, Cambridge, Massachusetts 02138, USA and Jena D-07745, Germany.; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.; Department of Human Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.; Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA.; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.; Pinhasi R; Department of Evolutionary Anthropology, University of Vienna, Vienna 1090, Austria.
Source
Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9518021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-5469 (Electronic) Linking ISSN: 10889051 NLM ISO Abbreviation: Genome Res Subsets: MEDLINE
Subject
Language
English
Abstract
Ancient DNA sampling methods-although optimized for efficient DNA extraction-are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive sampling of the skeletal remains from which ancient DNA is obtained. Due to a particularly high concentration of endogenous DNA, the cementum of tooth roots is often targeted for ancient DNA sampling, but destructive sampling methods of the cementum often result in the loss of at least one entire root. Here, we present a minimally destructive method for extracting ancient DNA from dental cementum present on the surface of tooth roots. This method does not require destructive drilling or grinding, and, following extraction, the tooth remains safe to handle and suitable for most morphological studies, as well as other biochemical studies, such as radiocarbon dating. We extracted and sequenced ancient DNA from 30 teeth (and nine corresponding petrous bones) using this minimally destructive extraction method in addition to a typical tooth sampling method. We find that the minimally destructive method can provide ancient DNA that is of comparable quality to extracts produced from teeth that have undergone destructive sampling processes. Further, we find that a rigorous cleaning of the tooth surface combining diluted bleach and UV light irradiation seems sufficient to minimize external contaminants usually removed through the physical removal of a superficial layer when sampling through regular powdering methods.
(© 2021 Harney et al.; Published by Cold Spring Harbor Laboratory Press.)
(© 2021 Harney et al.; Published by Cold Spring Harbor Laboratory Press.)