학술논문
Prognostic Factors Associating with Pro-oxidant-antioxidant Balance; Neutrophils to Lymphocytes Ratio, Vitamin D, Heat Shock Protein 27, and Red Cell Distribution Width.
Document Type
Academic Journal
Author
Ghazizadeh H; Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Department of Nutrition, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Mirinezhad MR; Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Seyedi SMR; Department of biology, Ferdowsi University of Mashhad, Faculty of Science, Mashhad, Iran.; Sadabadi F; Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Ahmadnezhad M; Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Jaberi N; Department of Medical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Pasdar A; Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Division of Applied Medicine, Medical School, University of Aberdeen, Aberdeen, UK.; Ferns GA; Division of Medical Education, Brighton & Sussex Medical School, Brighton, Sussex, UK.; Esmaily H; Department of Biostatistics and Epidemiology, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran.; Ghayour-Mobarhan M; Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.
Source
Publisher: Elsevier Science Country of Publication: United States NLM ID: 9312706 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5487 (Electronic) Linking ISSN: 01884409 NLM ISO Abbreviation: Arch Med Res Subsets: MEDLINE
Subject
Language
English
Abstract
Background: Several chronic diseases are mediated by oxidative stress. Oxidative stress affects cell morphology and function and is associated with alterations in the serum protein component. In the current study, we analyzed four individual prognostic factors associating with serum Pro-Oxidant-Antioxidant Balance (PAB): neutrophil to lymphocyte ratio (NLR), Vitamin D, anti-heat shock protein 27 (anti-hsp27) antibody titer, and red blood cell distribution width (RDW) to evaluate them as the potential prognostic markers. In the current study, we attempted to investigate the relationship between serum PAB, RDW, NLR, serum vitamin D and anti-hsp27 concentration.
Methods: A total of 852 participants (438 males and 414 females) aged 47.64 ± 7.77 years were recruited in a cross-sectional study based on the Mashhad stroke and heart atherosclerotic disorders (MASHAD) cohort study data. Hematological parameters, and vitamin D, PAB and anti-hsp27 antibody titers were measured using the Sysmex auto analyzer system and enzyme-linked immune sorbent assay (ELISA), respectively.
Results: The results showed a significant correlation between Vitamin D and anti-hsp27 antibody titers (r = -0.13 and p <0.001) as well as between RDW and serum PAB (r = 0.120 and p <0.001). Moreover, we found that serum PAB was positively associated with serum anti hsp27 antibody titers. The results showed increasing 1 unit of serum vitamin D can cause 3% decreases in anti hsp 27 values (OR = 0.97; CI 95% (0.96-0.99); p = 0.004). While this association was not significant for RDW, NLR and PAB (p >0.05) we found a significant association between serum PAB and serum anti hsp-27 antibody titers. Subjects with PAB levels 36.31-82.63 had a higher risk (1.83 fold) of having an increased anti-hsp27 antibody titers in comparison to the reference group (PAB level <36.31) (OR = 1.83 (95% CI = 1.33-2.52), p <0.001).
Conclusion: The present study shows that serum vitamin D can be associated with reduction in inflammatory status probably by decreasing levels of serum anti-hsp27 antibody titers, reduction in oxidative stress and therefore may reduce the risk of cardiovascular diseases. Anti-hsp27 antibody titers are associated with oxidative stress through the serum PAB, therefore these factors may be of prognostic values in detecting oxidative stress and risk of atherosclerosis. The evaluation of these factors in a larger population may help further confirm these findings.
(Copyright © 2020 IMSS. Published by Elsevier Inc. All rights reserved.)
Methods: A total of 852 participants (438 males and 414 females) aged 47.64 ± 7.77 years were recruited in a cross-sectional study based on the Mashhad stroke and heart atherosclerotic disorders (MASHAD) cohort study data. Hematological parameters, and vitamin D, PAB and anti-hsp27 antibody titers were measured using the Sysmex auto analyzer system and enzyme-linked immune sorbent assay (ELISA), respectively.
Results: The results showed a significant correlation between Vitamin D and anti-hsp27 antibody titers (r = -0.13 and p <0.001) as well as between RDW and serum PAB (r = 0.120 and p <0.001). Moreover, we found that serum PAB was positively associated with serum anti hsp27 antibody titers. The results showed increasing 1 unit of serum vitamin D can cause 3% decreases in anti hsp 27 values (OR = 0.97; CI 95% (0.96-0.99); p = 0.004). While this association was not significant for RDW, NLR and PAB (p >0.05) we found a significant association between serum PAB and serum anti hsp-27 antibody titers. Subjects with PAB levels 36.31-82.63 had a higher risk (1.83 fold) of having an increased anti-hsp27 antibody titers in comparison to the reference group (PAB level <36.31) (OR = 1.83 (95% CI = 1.33-2.52), p <0.001).
Conclusion: The present study shows that serum vitamin D can be associated with reduction in inflammatory status probably by decreasing levels of serum anti-hsp27 antibody titers, reduction in oxidative stress and therefore may reduce the risk of cardiovascular diseases. Anti-hsp27 antibody titers are associated with oxidative stress through the serum PAB, therefore these factors may be of prognostic values in detecting oxidative stress and risk of atherosclerosis. The evaluation of these factors in a larger population may help further confirm these findings.
(Copyright © 2020 IMSS. Published by Elsevier Inc. All rights reserved.)