학술논문
Epithelial cell adhesion molecule (EpCAM) from pleural fluid cell lysates is a highly accurate diagnostic biomarker of adenocarcinomatous effusions.
Document Type
Academic Journal
Author
Porcel JM; Pleural Medicine Unit, Department of Internal Medicine, Arnau de Vilanova University Hospital, Lleida, Spain.; Research Group of Cancer Biomarkers, IRBLleida, Lleida, Spain.; Esquerda A; Research Group of Cancer Biomarkers, IRBLleida, Lleida, Spain.; Department of Laboratory Medicine, Arnau de Vilanova University Hospital, Lleida, Spain.; Bielsa S; Pleural Medicine Unit, Department of Internal Medicine, Arnau de Vilanova University Hospital, Lleida, Spain.; Research Group of Cancer Biomarkers, IRBLleida, Lleida, Spain.; Novell A; Research Group of Cancer Biomarkers, IRBLleida, Lleida, Spain.; Sorolla MA; Research Group of Cancer Biomarkers, IRBLleida, Lleida, Spain.; Gatius S; Department of Pathology, Arnau de Vilanova University Hospital, Lleida, Spain.; Zamora C; Department of Immunology, Institut de Recerca, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.; Vidal S; Department of Immunology, Institut de Recerca, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.; Salud A; Research Group of Cancer Biomarkers, IRBLleida, Lleida, Spain.; Department of Medical Oncology, Arnau de Vilanova University Hospital, Lleida, Spain.
Source
Publisher: Blackwell Science Country of Publication: Australia NLM ID: 9616368 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1440-1843 (Electronic) Linking ISSN: 13237799 NLM ISO Abbreviation: Respirology Subsets: MEDLINE
Subject
Language
English
Abstract
Background and Objective: The discovery of highly accurate pleural fluid (PF) biomarkers of malignancy remains elusive. We assessed the operating characteristics of the PF epithelial cell adhesion molecule (EpCAM), claudin 4 (CL4) and human epididymis protein 4 (HE4) as potential markers of epithelial malignancies.
Methods: The three markers were quantified by immunoassays in the supernatants (s) and cell lysates (cl) of 175 PF samples. The cut-off values with 100% specificity were selected for malignant-benign discrimination. An immunocytochemical staining index score for each marker was also evaluated on PF cell blocks. The resulting best biomarker was further validated in two independent populations of 73 and 48 patients with pleural effusions (PE).
Results: An EpCAM(cl) >98 pg/g total lysate protein yielded 75% sensitivity, 100% specificity, negative likelihood ratio of 0.25 and area under the curve of 0.94 for labelling adenocarcinomatous effusions. Sensitivity reached 88% if EpCAM(cl) was combined with EpCAM immunostaining. One-third or more of the malignant effusions exhibiting a false-negative cytological fluid examination were correctly classified by EpCAM(cl) concentrations. Immunoassays for CL4 and HE4 were diagnostically useless.
Conclusion: EpCAM(cl) is a new biomarker of adenocarcinomatous PE with meaningful discriminating properties.
(© 2019 Asian Pacific Society of Respirology.)
Methods: The three markers were quantified by immunoassays in the supernatants (s) and cell lysates (cl) of 175 PF samples. The cut-off values with 100% specificity were selected for malignant-benign discrimination. An immunocytochemical staining index score for each marker was also evaluated on PF cell blocks. The resulting best biomarker was further validated in two independent populations of 73 and 48 patients with pleural effusions (PE).
Results: An EpCAM(cl) >98 pg/g total lysate protein yielded 75% sensitivity, 100% specificity, negative likelihood ratio of 0.25 and area under the curve of 0.94 for labelling adenocarcinomatous effusions. Sensitivity reached 88% if EpCAM(cl) was combined with EpCAM immunostaining. One-third or more of the malignant effusions exhibiting a false-negative cytological fluid examination were correctly classified by EpCAM(cl) concentrations. Immunoassays for CL4 and HE4 were diagnostically useless.
Conclusion: EpCAM(cl) is a new biomarker of adenocarcinomatous PE with meaningful discriminating properties.
(© 2019 Asian Pacific Society of Respirology.)