학술논문
Multi-species host range of staphylococcal phages isolated from wastewater.
Document Type
Academic Journal
Author
Göller PC; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Elsener T; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Lorgé D; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Radulovic N; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Bernardi V; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Naumann A; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Amri N; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Khatchatourova E; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Coutinho FH; Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, San Juan de Alicante, Spain.; Loessner MJ; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland.; Gómez-Sanz E; Institute of Food, Nutrition and Health, ETH Zurich, 8092, Zurich, Switzerland. elena.gomez@hest.ethz.ch.; Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain. elena.gomez@hest.ethz.ch.
Source
Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
Subject
Language
English
Abstract
The host range of bacteriophages defines their impact on bacterial communities and genome diversity. Here, we characterize 94 novel staphylococcal phages from wastewater and establish their host range on a diversified panel of 117 staphylococci from 29 species. Using this high-resolution phage-bacteria interaction matrix, we unveil a multi-species host range as a dominant trait of the isolated staphylococcal phages. Phage genome sequencing shows this pattern to prevail irrespective of taxonomy. Network analysis between phage-infected bacteria reveals that hosts from multiple species, ecosystems, and drug-resistance phenotypes share numerous phages. Lastly, we show that phages throughout this network can package foreign genetic material enclosing an antibiotic resistance marker at various frequencies. Our findings indicate a weak host specialism of the tested phages, and therefore their potential to promote horizontal gene transfer in this environment.
(© 2021. The Author(s).)
(© 2021. The Author(s).)