학술논문
Host genetic factors and clinical parameters influencing the occult hepatitis C virus infection in patients on chronic hemodialysis: Is it still a controversial infection?
Document Type
Academic Journal
Author
Ayadi A; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.; Nafari AH; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.; Sakhaee F; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.; Rajabi K; Department of Microbiology, Islamic Azad University, Pharmaceutical, Sciences Branch, Tehran, Iran.; Ghaderi Y; Islamic Azad University Central Tehran Branch, Faculty of Basic Science, Tehran, Iran.; Rahimi Jamnani F; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.; Vaziri F; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.; Siadat SD; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.; Fateh A; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
Source
Publisher: Blackwell Publishing Country of Publication: Netherlands NLM ID: 9711801 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1386-6346 (Print) Linking ISSN: 13866346 NLM ISO Abbreviation: Hepatol Res Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
1386-6346
Abstract
Aim: The presence of occult hepatitis C virus (HCV) infection (OCI) is still controversial, however, this infection cannot be ignored. Therefore, the current study aimed at assessing the OCI frequency in patients on chronic hemodialysis (CHD) and also evaluating the association between OCI incidence with clinical parameters and interferon lambda 3/4 (IFNL3/4) gene polymorphisms.
Methods: A total of 515 patients on CHD and HCV negative markers were selected. Plus- and minus-stranded HCV-RNA was tested in peripheral blood mononuclear cell samples by reverse transcription-polymerase chain reaction and then genotyped using the restriction fragment length polymorphism method.
Results: The frequency of OCI was 11.3% in patients on CHD. Among 58 patients with OCI, 25.8%, 62.1%, and 12.1% were infected with HCV-1a, HCV-1b, and HCV-3a, respectively. The mean alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels were 31.9 ± 24.8, 32.3 ± 19.1, and 171.6 ± 88.9, respectively. None of the patients with OCI had a history of liver disease. Multivariate logistic regression analysis indicated that cholesterol, triglyceride, low-density lipoprotein, 25-hydroxyvitamin D, platelets, duration of hemodialysis, HCV subtypes, IFNL3 rs12979860 TT, IFNL3 rs8099917 GG, IFNL3 rs12980275 GG, and IFNL4 ss469415590 ΔG/ΔG genotypes were associated with OCI.
Conclusion: There was a moderate prevalence of OCI in Iranian patients on CHD. The current study findings indicated that this infection was associated with clinical parameters and unfavorable genotypes of IFNL3 single nucleotide polymorphisms and IFNL4 ss469415590. Further studies are required to determine the correlation between OCI incidence with clinical parameters and host genetic factors.
(© 2019 The Japan Society of Hepatology.)
Methods: A total of 515 patients on CHD and HCV negative markers were selected. Plus- and minus-stranded HCV-RNA was tested in peripheral blood mononuclear cell samples by reverse transcription-polymerase chain reaction and then genotyped using the restriction fragment length polymorphism method.
Results: The frequency of OCI was 11.3% in patients on CHD. Among 58 patients with OCI, 25.8%, 62.1%, and 12.1% were infected with HCV-1a, HCV-1b, and HCV-3a, respectively. The mean alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels were 31.9 ± 24.8, 32.3 ± 19.1, and 171.6 ± 88.9, respectively. None of the patients with OCI had a history of liver disease. Multivariate logistic regression analysis indicated that cholesterol, triglyceride, low-density lipoprotein, 25-hydroxyvitamin D, platelets, duration of hemodialysis, HCV subtypes, IFNL3 rs12979860 TT, IFNL3 rs8099917 GG, IFNL3 rs12980275 GG, and IFNL4 ss469415590 ΔG/ΔG genotypes were associated with OCI.
Conclusion: There was a moderate prevalence of OCI in Iranian patients on CHD. The current study findings indicated that this infection was associated with clinical parameters and unfavorable genotypes of IFNL3 single nucleotide polymorphisms and IFNL4 ss469415590. Further studies are required to determine the correlation between OCI incidence with clinical parameters and host genetic factors.
(© 2019 The Japan Society of Hepatology.)