학술논문
Probing the interaction of silver nanoparticles with tau protein and neuroblastoma cell line as nervous system models.
Document Type
Academic Journal
Author
Rahmani S; a Faculty of Advance Science and Technology, Department of Cell and Molecular Biology , Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS) , Tehran , Iran.; Mogharizadeh L; b Department of Cell and Molecular Biology , Islamic Azad University, Central Tehran Branch , Tehran , Iran.; Attar F; c Department of Biology, Faculty of Food Industry & Agriculture , Standard Research Institute (SRI) , Karaj , Iran.; Rezayat SM; d Department of Pharmacology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.; e Department of Medical Nanotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.; Mousavi SE; d Department of Pharmacology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.; Falahati M; f Faculty of Advance Science and Technology, Department of Nanotechnology , Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS) , Tehran , Iran.
Source
Publisher: Taylor & Francis Country of Publication: England NLM ID: 8404176 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-0254 (Electronic) Linking ISSN: 07391102 NLM ISO Abbreviation: J Biomol Struct Dyn Subsets: MEDLINE
Subject
Language
English
Abstract
Interestingly pharmaceutical sciences are using nanoparticles (NPs) to design and develop nanomaterials-based drugs. However, up to recently, it has not been well realized that NPs themselves may impose risks to the biological systems. In this study, the interaction of silver nanoparticles (AgNPs) with tau protein and SH-SY5Y neuroblastoma cell line, as potential nervous system models, was examined with a range of techniques including intrinsic fluorescence spectroscopy, circular dichroism (CD) spectroscopy, 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and acridine orange/ethidium bromide (AO/EB) dual staining method. Fluorescence study showed that AgNPs with a diameter of around 10-20 nm spontaneously form a static complex with tau protein via hydrogen bonds and van der Waals interactions. CD experiment revealed that AgNPs did not change the random coil structure of tau protein. Moreover, AgNPs showed to induce SH-SY5Y neuroblastoma cell mortality through fragmentation of DNA which is a key feature of apoptosis. In conclusion, AgNPs may induce slight changes on the tau protein structure. Also, the concentration of AgNPs is the main factor which influences their cytotoxicity. Since, all adverse effects of NPs are not well detected, so probably additional more specific testing would be needed.