학술논문
Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny.
Document Type
Academic Journal
Author
Pérez-Martín F; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, 04120, Almería, Spain.; Yuste-Lisbona FJ; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, 04120, Almería, Spain.; Pineda B; Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, 46022, Valencia, Spain.; García-Sogo B; Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, 46022, Valencia, Spain.; Olmo ID; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcón (Madrid), Spain.; de Dios Alché J; Departamento de Bioquímica, Biología Celular y Molecular de Plantas, EEZ-CSIC, 18008, Granada, Spain.; Egea I; Departamento de Biología del Estrés y Patología Vegetal, CEBAS-CSIC, 30100, Espinardo-Murcia, Spain.; Flores FB; Departamento de Biología del Estrés y Patología Vegetal, CEBAS-CSIC, 30100, Espinardo-Murcia, Spain.; Piñeiro M; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcón (Madrid), Spain.; Jarillo JA; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcón (Madrid), Spain.; Angosto T; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, 04120, Almería, Spain.; Capel J; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, 04120, Almería, Spain.; Moreno V; Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, 46022, Valencia, Spain.; Lozano R; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, 04120, Almería, Spain.
Source
Publisher: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology Country of Publication: England NLM ID: 9207397 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-313X (Electronic) Linking ISSN: 09607412 NLM ISO Abbreviation: Plant J
Subject
Language
English
Abstract
Pollen development is a crucial step in higher plants, which not only makes possible plant fertilization and seed formation, but also determines fruit quality and yield in crop species. Here, we reported a tomato T-DNA mutant, pollen deficient1 (pod1), characterized by an abnormal anther development and the lack of viable pollen formation, which led to the production of parthenocarpic fruits. Genomic analyses and the characterization of silencing lines proved that pod1 mutant phenotype relies on the tomato SlMED18 gene encoding the subunit 18 of Mediator multi-protein complex involved in RNA polymerase II transcription machinery. The loss of SlMED18 function delayed tapetum degeneration, which resulted in deficient microspore development and scarce production of viable pollen. A detailed histological characterization of anther development proved that changes during microgametogenesis and a significant delay in tapetum degeneration are associated with a high proportion of degenerated cells and, hence, should be responsible for the low production of functional pollen grains. Expression of pollen marker genes indicated that SlMED18 is essential for the proper transcription of a subset of genes specifically required to pollen formation and fruit development, revealing a key role of SlMED18 in male gametogenesis of tomato. Additionally, SlMED18 is able to rescue developmental abnormalities of the Arabidopsis med18 mutant, indicating that most biological functions have been conserved in both species.
(© 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd.)
(© 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd.)