학술논문
Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany.
Document Type
Academic Journal
Author
Bauswein M; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, Germany.; Eidenschink L; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, Germany.; Knoll G; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, Germany.; Neumann B; Department of Neurology, Donau-Isar-Klinikum Deggendorf, 94469 Deggendorf, Germany.; Department of Neurology, University of Regensburg, Bezirksklinikum, 93053 Regensburg, Germany.; Angstwurm K; Department of Neurology, University of Regensburg, Bezirksklinikum, 93053 Regensburg, Germany.; Zoubaa S; Department of Neuropathology, University Hospital Regensburg, 93053 Regensburg, Germany.; Riemenschneider MJ; Department of Neuropathology, University Hospital Regensburg, 93053 Regensburg, Germany.; Lampl BMJ; Regensburg Department of Public Health, 93059 Regensburg, Germany.; Department of Epidemiology and Preventive Medicine, University of Regensburg, 93053 Regensburg, Germany.; Pregler M; Regensburg Department of Public Health, 93059 Regensburg, Germany.; Niller HH; Institute of Medical Microbiology and Hygiene, University of Regensburg, 93053 Regensburg, Germany.; Jantsch J; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, Germany.; Institute for Medical Microbiology, Immunology and Hygiene, University Hospital Cologne and Faculty of Medicine, University of Cologne, 50935 Cologne, Germany.; Gessner A; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, Germany.; Institute of Medical Microbiology and Hygiene, University of Regensburg, 93053 Regensburg, Germany.; Eberhardt Y; Center for Clinical Studies, University Hospital Regensburg, 93053 Regensburg, Germany.; Huppertz G; Center for Clinical Studies, University Hospital Regensburg, 93053 Regensburg, Germany.; Schramm T; Center for Clinical Studies, University Hospital Regensburg, 93053 Regensburg, Germany.; Kühn S; Center for Clinical Studies, University Hospital Regensburg, 93053 Regensburg, Germany.; Koller M; Center for Clinical Studies, University Hospital Regensburg, 93053 Regensburg, Germany.; Drasch T; Department of Nephrology, University Hospital Regensburg, 93053 Regensburg, Germany.; Ehrl Y; Department of Nephrology, University Hospital Regensburg, 93053 Regensburg, Germany.; Banas B; Department of Nephrology, University Hospital Regensburg, 93053 Regensburg, Germany.; Offner R; Institute of Clinical Chemistry and Laboratory Medicine, Department of Transfusion Medicine, University Hospital Regensburg, 93053 Regensburg, Germany.; Schmidt B; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, Germany.; Institute of Medical Microbiology and Hygiene, University of Regensburg, 93053 Regensburg, Germany.; Banas MC; Department of Nephrology, University Hospital Regensburg, 93053 Regensburg, Germany.
Source
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
Subject
Language
English
Abstract
More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study ("BoDV-1 after solid-organ transplantation") to assess whether there are undetected oligo- or asymptomatic courses of infection. A total of 216 healthy blood donors and 280 outpatients after solid organ transplantation were screened by a recombinant BoDV-1 ELISA followed by an indirect immunofluorescence assay (iIFA) as confirmatory test. For comparison, 288 serum and 258 cerebrospinal fluid (CSF) samples with a request for tick-borne encephalitis (TBE) diagnostics were analyzed for BoDV-1 infections. ELISA screening reactivity rates ranged from 3.5% to 18.6% depending on the cohort and the used ELISA antigen, but only one sample of a patient from the cohort with requested TBE diagnostics was confirmed to be positive for anti-BoDV-1-IgG by iIFA. In addition, the corresponding CSF sample of this patient with a three-week history of severe neurological disease tested positive for BoDV-1 RNA. Due to the iIFA results, all other results were interpreted as false-reactive in the ELISA screening. By linear serological epitope mapping, cross-reactions with human and bacterial proteins were identified as possible underlying mechanism for the false-reactive ELISA screening results. In conclusion, no oligo- or asymptomatic infections were detected in the studied cohorts. Serological tests based on a single recombinant BoDV-1 antigen should be interpreted with caution, and an iIFA should always be performed in addition.