학술논문
Interindividual variation in human cortical cell type abundance and expression.
Document Type
Academic Journal
Author
Johansen N; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Somasundaram S; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Travaglini KJ; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Yanny AM; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Shumyatcher M; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Casper T; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Cobbs C; Swedish Neuroscience Institute, Seattle,WA 98122, USA.; Dee N; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Ellenbogen R; Department of Neurological Surgery, University of Washington, Seattle, WA 98104, USA.; Ferreira M; Department of Neurological Surgery, University of Washington, Seattle, WA 98104, USA.; Goldy J; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Guzman J; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Gwinn R; Swedish Neuroscience Institute, Seattle,WA 98122, USA.; Hirschstein D; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Jorstad NL; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Keene CD; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98104, USA.; Ko A; Department of Neurological Surgery, University of Washington, Seattle, WA 98104, USA.; Levi BP; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Ojemann JG; Department of Neurological Surgery, University of Washington, Seattle, WA 98104, USA.; Pham T; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Shapovalova N; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Silbergeld D; Department of Neurological Surgery, University of Washington, Seattle, WA 98104, USA.; Sulc J; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Torkelson A; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Tung H; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Smith K; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Lein ES; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Bakken TE; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Hodge RD; Allen Institute for Brain Science, Seattle, WA 98109, USA.; Miller JA; Allen Institute for Brain Science, Seattle, WA 98109, USA.
Source
Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
Subject
Language
English
Abstract
Single-cell transcriptomic studies have identified a conserved set of neocortical cell types from small postmortem cohorts. We extended these efforts by assessing cell type variation across 75 adult individuals undergoing epilepsy and tumor surgeries. Nearly all nuclei map to one of 125 robust cell types identified in the middle temporal gyrus. However, we found interindividual variance in abundances and gene expression signatures, particularly in deep-layer glutamatergic neurons and microglia. A minority of donor variance is explainable by age, sex, ancestry, disease state, and cell state. Genomic variation was associated with expression of 150 to 250 genes for most cell types. This characterization of cellular variation provides a baseline for cell typing in health and disease.