학술논문

Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging.
Document Type
Academic Journal
Author
Quardokus EM; Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.; Saunders DC; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.; Vanderbilt Diabetes Center, Vanderbilt University School of Medicine, Nashville, TN, USA.; McDonough E; GE Research, Niskayuna, NY, USA.; Hickey JW; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.; Werlein C; Institute of Pathology, Hannover Medical School, Hannover, Germany.; Surrette C; GE Research, Niskayuna, NY, USA.; Rajbhandari P; Department of Biological Sciences, Columbia University, New York, NY, USA.; Casals AM; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.; Department of Bioengineering, Stanford University, Stanford, CA, USA.; Tian H; Department of Chemistry, Pennsylvania State University, University Park, PA, USA.; Lowery L; GE Research, Niskayuna, NY, USA.; Neumann EK; Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.; Department of Chemistry, University of California Davis, Davis, CA, USA.; Björklund F; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.; Neelakantan TV; Department of Chemistry, Columbia University, New York, NY, USA.; Croteau J; Department of Business Development, BioLegend Inc., San Diego, CA, USA.; Wiblin AE; Department of Research and Development, Abcam PLC, Discovery Drive, Cambridge Biomedical Campus, Cambridge, UK.; Fisher J; Department of Research and Development, Cell Signaling Technology, Inc., Danvers, MA, USA.; Livengood AJ; Department of Protein and Cell Analysis, Thermo Fisher Scientific, Carlsbad, CA, USA.; Dowell KG; Akoya Biosciences, Marlborough, MA, USA.; Silverstein JC; Department of Biomedical Informatics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Spraggins JM; Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.; Department of Chemistry, Vanderbilt University, Nashville, TN, USA.; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.; Pryhuber GS; Department of Pediatrics, University of Rochester Medical Center, Rochester, NY, USA.; Deutsch G; Department of Pathology, University of Washington Medical Center, Seattle, WA, USA.; Ginty F; GE Research, Niskayuna, NY, USA.; Nolan GP; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.; Melov S; Buck Institute for Research on Aging, Novato, CA, USA.; Jonigk D; Institute of Pathology, Hannover Medical School, Hannover, Germany.; German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.; Institute of Pathology, RWTH University of Aachen, Aachen, Germany.; Caldwell MA; Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.; Vlachos IS; Spatial Technologies Unit, Harvard Medical School Initiative for RNA Medicine, Beth Israel Deaconess Medical Center, Boston, MA, USA.; Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA.; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Muller W; Miltenyi Biotec B.V. and Co. KG, Bergisch Gladbach, Germany.; Division of Infection, Immunity and Respiratory Medicine, University of Manchester, Manchester, UK.; Gehlenborg N; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.; Stockwell BR; Department of Biological Sciences, Columbia University, New York, NY, USA.; Department of Chemistry, Columbia University, New York, NY, USA.; Lundberg E; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.; Department of Bioengineering, Stanford University, Stanford, CA, USA.; Snyder MP; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.; Germain RN; Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH, Bethesda, MD, USA.; Camarillo JM; Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.; Inotiv, Nashville, TN, USA.; Kelleher NL; Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.; Börner K; Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.; Radtke AJ; Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH, Bethesda, MD, USA. andrea.radtke@nih.gov.
Source
Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
Subject
Language
English
Abstract
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.
(© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.)