학술논문
Efficient granulocyte collection method using high concentrations of medium molecular weight hydroxyethyl starch.
Document Type
Academic Journal
Author
Kondo T; Division of Transfusion, Okayama University Hospital, Okayama, Japan.; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Fujii K; Division of Clinical Laboratory, Okayama University Hospital, Okayama, Japan.; Fujii N; Division of Transfusion, Okayama University Hospital, Okayama, Japan.; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Sumii Y; Division of Transfusion, Okayama University Hospital, Okayama, Japan.; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Urata T; Division of Transfusion, Okayama University Hospital, Okayama, Japan.; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Kimura M; Division of Transfusion, Okayama University Hospital, Okayama, Japan.; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Matsuda M; Division of Transfusion, Okayama University Hospital, Okayama, Japan.; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Ikegawa S; Division of Transfusion, Okayama University Hospital, Okayama, Japan.; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Washio K; Department of Pediatrics/Pediatric Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Fujiwara H; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Asada N; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Ennishi D; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Center for Comprehensive Genomic Medicine, Okayama University Hospital, Okayama, Japan.; Nishimori H; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Matsuoka KI; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.; Otsuka F; Division of Clinical Laboratory, Okayama University Hospital, Okayama, Japan.; Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.; Maeda Y; Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.
Source
Publisher: American Association Of Blood Banks Country of Publication: United States NLM ID: 0417360 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1537-2995 (Electronic) Linking ISSN: 00411132 NLM ISO Abbreviation: Transfusion Subsets: MEDLINE
Subject
Language
English
Abstract
Background: Granulocyte transfusion therapy is a rational therapeutic option for patients with prolonged, severe neutropenia. Although high molecular weight hydroxyethyl starch (hHES) facilitates the separation of red blood cells during granulocyte collection, renal dysfunction has been noted as a potential side effect. HES130/0.4 (Voluven®) is a medium molecular weight HES (mHES) with superior safety profiles compared to hHES. Although HES130/0.4 is reportedly effective in the collection of granulocytes, we lack studies comparing the efficiency of granulocyte collection using HES130/0.4 and hHES.
Study Design and Methods: We retrospectively collected the data from 60 consecutive apheresis procedures performed on 40 healthy donors at the Okayama University Hospital between July 2013 and December 2021. All procedures were performed using the Spectra Optia system. Based on the HES130/0.4 concentration in the separation chamber, granulocyte collection methods using HES130/0.4 were classified into m0.46, m0.44, m0.37, and m0.8 groups. We used HES130/0.4 and hHES groups to compare the various sample collection methods.
Results: The median granulocyte collection efficiency (CE) was approximately 24.0% and 28.1% in the m0.8 and hHES groups, respectively, which were significantly higher than those in the m0.46, m0.44, and m0.37 groups. One month following granulocyte collection with HES130/0.4, no significant changes were observed in serum creatinine levels compared to those before the donation.
Conclusion: Therefore, we propose a granulocyte collection approach employing HES130/0.4, which is comparable to the use of hHES in terms of the granulocyte CE. A high concentration of HES130/0.4 in the separation chamber was considered crucial for granulocyte collection.
(© 2023 AABB.)
Study Design and Methods: We retrospectively collected the data from 60 consecutive apheresis procedures performed on 40 healthy donors at the Okayama University Hospital between July 2013 and December 2021. All procedures were performed using the Spectra Optia system. Based on the HES130/0.4 concentration in the separation chamber, granulocyte collection methods using HES130/0.4 were classified into m0.46, m0.44, m0.37, and m0.8 groups. We used HES130/0.4 and hHES groups to compare the various sample collection methods.
Results: The median granulocyte collection efficiency (CE) was approximately 24.0% and 28.1% in the m0.8 and hHES groups, respectively, which were significantly higher than those in the m0.46, m0.44, and m0.37 groups. One month following granulocyte collection with HES130/0.4, no significant changes were observed in serum creatinine levels compared to those before the donation.
Conclusion: Therefore, we propose a granulocyte collection approach employing HES130/0.4, which is comparable to the use of hHES in terms of the granulocyte CE. A high concentration of HES130/0.4 in the separation chamber was considered crucial for granulocyte collection.
(© 2023 AABB.)