학술논문
Investigation of Epstein-Barr virus, Cytomegalovirus, Human herpesvirus 6, and Polyoma viruses (JC virus, BK virus) among Gastric cancer patients: A cross sectional study.
Document Type
Academic Journal
Author
Soltani S; Students' Scientific Research Center Tehran University of Medical Sciences Tehran Iran.; Department of Virology School of Public Health, Tehran University of Medical Sciences Tehran Iran.; Farahani A; Molecular and Medicine Research Center Khomein University of Medical Sciences Khomein Iran.; Department of Medical Laboratory Sciences Khomein University of Medical Sciences Khomein Iran.; Shahbahrami R; Students' Scientific Research Center Tehran University of Medical Sciences Tehran Iran.; Shateri Z; Student Research Committee Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran.; Emadi MS; Department of Medical Laboratory Sciences School of Allied Medical Sciences, Tehran University of Medical Sciences Tehran Iran.; Pakzad R; Department of Epidemiology Faculty of Health, Ilam University of Medical Sciences Ilam Iran.; Student Research Committee Ilam University of Medical Sciences Ilam Iran.; Lotfi M; Department of Pathology School of Medicine, Amir Alam Hospital, Tehran University of Medical Sciences Tehran Iran.; Asanjarani B; Department of Internal Medicine School of Medicine, Amir Alam Hospital, Tehran University of Medical Sciences Tehran Iran.; Rasti A; Department of Medical Surgery and Basic Sciences Faculty of Nursing and Midwifery Tehran Iran.; Erfani Y; Department of Medical Laboratory Sciences School of Allied Medical Sciences, Tehran University of Medical Sciences Tehran Iran.; Siri G; Department of Internal Medicine Amir Alam Hospital, School of Medicine, Tehran University of Medical Sciences Tehran Iran.
Source
Publisher: John Wiley and Sons, Inc Country of Publication: United States NLM ID: 101728855 Publication Model: eCollection Cited Medium: Internet ISSN: 2398-8835 (Electronic) Linking ISSN: 23988835 NLM ISO Abbreviation: Health Sci Rep Subsets: PubMed not MEDLINE
Subject
Language
English
Abstract
Background and Aims: Gastric cancer is a significant global issue with a high death rate. This malignancy could be associated with several viral agents such as EBV, CMV, HHV-6, JCV, and BKV.
Objective: Evaluation of EBV, CMV, HHV-6 ,and JCV, BKV frequency among gastric cancer patients.
Methods: In this cross-sectional study, a total number of 60 gastric cancer specimens (32 male, 28 female) were retrieved from the pathology lab. Formalin-fixed paraffin-embedded tissue was used for molecular testing. DNA was extracted from samples, according to protocol, and used for PCR reaction. Polymerase chain reactions were used to assess CMV, EBV, HHV-6, JCV, and BKV frequency.
Results and Conclusion: The mean age of the participants was 61 years and 53.3% (32) of the participants were Male. A total number of 5 samples (8.34%) were infected with viral agents. Four male gastric samples were infected with EBV (6.67%) and only one female sample contained the BKV genome (1.67%). Totally 8.34% of the samples were infected with EBV and BKV. The CMV, HHV-6, and JCV genome was not detected in the samples. In conclusion, the presence of two viral agents including EBV and BKV among male and female samples respectively, and the genome of other viruses were not detected.
Competing Interests: The authors declare no conflict of interest.
(© 2024 The Authors. Health Science Reports published by Wiley Periodicals LLC.)
Objective: Evaluation of EBV, CMV, HHV-6 ,and JCV, BKV frequency among gastric cancer patients.
Methods: In this cross-sectional study, a total number of 60 gastric cancer specimens (32 male, 28 female) were retrieved from the pathology lab. Formalin-fixed paraffin-embedded tissue was used for molecular testing. DNA was extracted from samples, according to protocol, and used for PCR reaction. Polymerase chain reactions were used to assess CMV, EBV, HHV-6, JCV, and BKV frequency.
Results and Conclusion: The mean age of the participants was 61 years and 53.3% (32) of the participants were Male. A total number of 5 samples (8.34%) were infected with viral agents. Four male gastric samples were infected with EBV (6.67%) and only one female sample contained the BKV genome (1.67%). Totally 8.34% of the samples were infected with EBV and BKV. The CMV, HHV-6, and JCV genome was not detected in the samples. In conclusion, the presence of two viral agents including EBV and BKV among male and female samples respectively, and the genome of other viruses were not detected.
Competing Interests: The authors declare no conflict of interest.
(© 2024 The Authors. Health Science Reports published by Wiley Periodicals LLC.)