학술논문
Differentiation of human umbilical cord mesenchymal stem cell into germ-like cell under effect of co-culture with testicular cell tissue.
Document Type
Academic Journal
Author
Majidi F; Department of Animal Physiology, Shahid Beheshti University, Tehran, Iran.; Bamehr H; Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.; Shalchian Z; Department of Food Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.; Kouchakian MR; Department of Anatomical Science, School of Medicine, Iran University of Medical Science, Tehran, Iran.; Mohammadzadeh N; Department of Molecular Medicine, Cancer Biomedical Center, Tehran, Iran.; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.; Khalili A; Department of Molecular Medicine, Cancer Biomedical Center, Tehran, Iran.; Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Source
Publisher: Wiley-Blackwell Country of Publication: Germany NLM ID: 7704218 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0264 (Electronic) Linking ISSN: 03402096 NLM ISO Abbreviation: Anat Histol Embryol Subsets: MEDLINE
Subject
Language
English
Abstract
Supplements produced by mouse testicular cells (mTCs) and the interaction between cells can increase the differentiation rate of human umbilical cord mesenchymal stem cells (hUCMSCs) into the germ-like cells. We studied the differentiation rate of hUCMSCs into the germ-like cells under effect of mTCs co-culturing. Isolated hUCMSCs from postpartum human umbilical cords were cultured. Then, the expression of mesenchymal (CD73, CD90 and CD105) and haematopoietic (CD34 and CD45) markers of hUCMSCs were confirmed by flow cytometry. Then, the hUCMSCs were cultured in four distinct groups: (a) control, (b) co-culture until D0, (c) co-culture until D5 and (d) co-culture until D10, in order to differentiate into the germ-like cells. After 10 days, the expression of OCT4, VASA, Fragilis and SYCP3 genes were examined by Real-Time qPCR. The flow cytometry indicated a high expression of mesenchymal markers and a low expression of haematopoietic markers (CD73:98.6%, CD90: 99.1%, CD105: 99.5%, CD34: 4.22% and CD45: 2.54%). The expression of OCT4 decreased during the time while the expression of VASA, Fragilis and SYCP3 markers increased in the co-culture with testicular cells (p value <.05). Co-culture with mTCs may be used as an effective method to differentiate hUCMSCs into germ-like cells.
(© 2020 Blackwell Verlag GmbH.)
(© 2020 Blackwell Verlag GmbH.)