학술논문
SerpinB3 administration protects liver against ischemia-reperfusion injury.
Document Type
Academic Journal
Author
Turato C; Department of Molecular Medicine, University of Pavia. cristian.turato@unipv.it.; Vairetti M; Departmentt of Internal Medicine and Therapeutics, University of Pavia. mariapia.vairetti@unipv.it.; Cagna M; Department of Internal Medicine and Therapeutics, University of Pavia. marta.cagna02@universitadipavia.it.; Biasiolo A; Department of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Padua. alessandra.biasiolo@unipd.it.; Ferrigno A; Department of Internal Medicine and Therapeutics, University of Pavia. andrea.ferrigno@unipv.it.; Quarta S; Department of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Padua. santina.quarta@unipd.it.; Ruvoletto M; Department of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Padua. mariagrazia.ruvoletto@unipd.it.; De Siervi S; Department of Molecular Medicine, University of Pavia. silvia.desiervi01@universitadipavia.it.; Pontisso P; Department of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Padua. patrizia@unipd.it.; Di Pasqua LG; Department of Internal Medicine and Therapeutics, University of Pavia. lauragiuseppina.dipasqua@unipv.it.
Source
Publisher: PagePress Publications Country of Publication: Italy NLM ID: 9207930 Publication Model: Electronic Cited Medium: Internet ISSN: 2038-8306 (Electronic) Linking ISSN: 1121760X NLM ISO Abbreviation: Eur J Histochem Subsets: MEDLINE
Subject
Language
English
Abstract
We have investigated the change in SerpinB3 during hepatic ischemia and the potential role of its antiprotease activity in cell protection by the administration of wild-type SerpinB3 (SerpinB3-WT) or active loop-deleted recombinant SerpinB3 protein (SerpinB3-D) in a rat model of ischemia (60 min)/reperfusion (60 min) (I/R). A time-dependent increase of SerpinB3, both at transcription and protein level, was found in ischemic livers after 60, 120 and 180 min. SerpinB3-WT decreased polymorphonuclear cell infiltration and serum enzymes and increased ATP when compared with I/R group. These events were not obtained using SerpinB3-D. No significant changes in both liver SerpinB3 mRNA and protein were found in all I/R groups considered. The present data show that the administration of SerpinB3-WT reduced the I/R injury and this effect appears to be dependent on its anti-protease activity.