학술논문
pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ).
Document Type
Academic Journal
Author
Boulais M; CNRS, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), IUEM, Plouzané 29280, France myrina.boulais@gmail.com myrina.boulais@univ-brest.fr.; Suquet M; Ifremer, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), Site expérimental d'Argenton, Landunvez 29840, France.; Arsenault-Pernet EJ; Ifremer, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), Site expérimental d'Argenton, Landunvez 29840, France.; Malo F; Ifremer, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), Site expérimental d'Argenton, Landunvez 29840, France.; Queau I; Ifremer, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), Site expérimental d'Argenton, Landunvez 29840, France.; Pignet P; Ifremer, LMEE, Centre de Bretagne, Plouzané 29280, France.; Ratiskol D; Ifremer, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), Site expérimental d'Argenton, Landunvez 29840, France.; Le Grand J; Ifremer, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), Site expérimental d'Argenton, Landunvez 29840, France.; Huber M; Ifremer, UMR 6539 Lemar (UBO-CNRS-IRD-Ifremer), Site expérimental d'Argenton, Landunvez 29840, France.; Cosson J; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany 38925, Czech Republic.
Source
Publisher: Company of Biologists Ltd Country of Publication: England NLM ID: 101578018 Publication Model: Electronic Cited Medium: Print ISSN: 2046-6390 (Print) Linking ISSN: 20466390 NLM ISO Abbreviation: Biol Open Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2046-6390
Abstract
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas The acidic pH of the gonad (5.82±0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH 4 Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH 4 Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na + -free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na + /H + exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5).
Competing Interests: Competing interestsThe authors declare no competing or financial interests.
(© 2018. Published by The Company of Biologists Ltd.)
Competing Interests: Competing interestsThe authors declare no competing or financial interests.
(© 2018. Published by The Company of Biologists Ltd.)