학술논문
The response of Microcystis aeruginosa strain MGK to a single or two consecutive H 2 O 2 applications.
Document Type
Academic Journal
Author
Daniel E; Plants and Environmental Sciences, the Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 9190401, Israel.; Weiss G; Plants and Environmental Sciences, the Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 9190401, Israel.; Murik O; The Yigal Allon Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, Migdal, Israel.; Sukenik A; The Yigal Allon Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, Migdal, Israel.; Lieman-Hurwitz J; Plants and Environmental Sciences, the Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 9190401, Israel.; Kaplan A; Plants and Environmental Sciences, the Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 9190401, Israel.
Source
Publisher: Society for Applied Microbiology and Blackwell Pub Country of Publication: United States NLM ID: 101499207 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1758-2229 (Electronic) Linking ISSN: 17582229 NLM ISO Abbreviation: Environ Microbiol Rep Subsets: MEDLINE
Subject
Language
English
Abstract
Various approaches have been proposed to control/eliminate toxic Microcystis sp. blooms including H 2 O 2 treatments. Earlier studies showed that pre-exposure of various algae to oxidative stress induced massive cell death when cultures were exposed to an additional H 2 O 2 treatment. We examined the vulnerability of exponential and stationary-phase Microcystis sp. strain MGK cultures to single and double H 2 O 2 applications. Stationary cultures show a much higher ability to decompose H 2 O 2 than younger cultures. Nevertheless, they are more sensitive to an additional H 2 O 2 dose given 1-6 h after the first one. Transcript analyses following H 2 O 2 application showed a fast rise in glutathione peroxidase abundance (227-fold within an hour) followed by a steep decline thereafter. Other genes potentially engaged in oxidative stress were far less affected. Metabolic-related genes were downregulated after H 2 O 2 treatments. Among those examined, the transcript level of prk (encoding phosphoribulose kinase) was the slowest to recover in agreement with the decline in photosynthetic rate revealed by fluorescence measurements. Our findings shed light on the response of Microcystis MGK to oxidative stress suggesting that two consecutive H 2 O 2 applications of low concentrations are far more effective in controlling Microcystis sp. population than a single dose of a higher concentration.
(© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.)
(© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.)